Department of Ophthalmology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, Sichuan, China.
Eur Rev Med Pharmacol Sci. 2019 Jan;23(1):378-388. doi: 10.26355/eurrev_201901_16786.
Age-related macular degeneration (AMD) is mainly characterized by dysfunction of retinal pigment epithelium (RPE) cells. This study aimed to investigate the protective effects of resveratrol on oxidative damaged RPE cells.
Human D407 cells were divided into normal control (NC), H2O2 treated (H2O2, treating with H2O2 at a final concentration of 200 mol/l) and resveratrol treatment groups (treating with resveratrol at a concentration of 12.5, 25, 50 and 100 mg/l). Malondialdehyde (MDA) and superoxide dismutase (SOD) activities were examined using enzyme-linked immunosorbent assay (ELISA). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and cell count kit-8 (CCK-8) were used to examine cell viability. Cell cycle phase distribution and apoptosis of D407 cells were evaluated using flow cytometry assay. B-cell lymphoma-2 (Bcl-2) and cleaved caspase 3 expression were detected using quantitative real-time PCR (qRT-PCR) and Western blot assay, respectively.
Resveratrol significantly decreased inhibitive ratios of D407 cell growth compared to that of H2O2 group (p<0.05). Resveratrol significantly increased SOD activity compared to that of H2O2 group (p<0.05). Resveratrol significantly reduced MDA activity compared to that of H2O2 group (p<0.05). Resveratrol affected cell cycle phase distribution of D407 cells compared to that of H2O2 group (p<0.05). Resveratrol significantly decreased the early stage and late stage apoptosis rates compared to that of H2O2 group (p<0.05). Resveratrol significantly enhanced Bcl-2 levels and decreased cleaved caspase 3 levels compared to that of H2O2 group (p<0.05).
Resveratrol protected against the oxidative damage of RPE cells by modulating SOD/MDA activity and activating Bcl-2 expression.
年龄相关性黄斑变性（AMD）主要表现为视网膜色素上皮（RPE）细胞功能障碍。本研究旨在探讨白藜芦醇对氧化损伤的 RPE 细胞的保护作用。
人 D407 细胞分为正常对照组（NC）、H2O2 处理组（H2O2，终浓度 200mol/L 的 H2O2 处理）和白藜芦醇处理组（分别用 12.5、25、50 和 100mg/L 的白藜芦醇处理）。采用酶联免疫吸附试验（ELISA）检测丙二醛（MDA）和超氧化物歧化酶（SOD）活性。3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四唑溴盐（MTT）和细胞计数试剂盒-8（CCK-8）检测细胞活力。采用流式细胞术检测 D407 细胞周期分布和凋亡。采用实时定量 PCR（qRT-PCR）和 Western blot 检测 B 细胞淋巴瘤-2（Bcl-2）和裂解型半胱天冬酶 3（caspase 3）的表达。
与 H2O2 组相比，白藜芦醇显著降低了 D407 细胞生长抑制率（p<0.05）。与 H2O2 组相比，白藜芦醇显著增加了 SOD 活性（p<0.05）。与 H2O2 组相比，白藜芦醇显著降低了 MDA 活性（p<0.05）。与 H2O2 组相比，白藜芦醇影响了 D407 细胞的细胞周期分布（p<0.05）。与 H2O2 组相比，白藜芦醇显著降低了早期和晚期凋亡率（p<0.05）。与 H2O2 组相比，白藜芦醇显著增加了 Bcl-2 水平，降低了 cleaved caspase 3 水平（p<0.05）。
白藜芦醇通过调节 SOD/MDA 活性和激活 Bcl-2 表达，对 RPE 细胞的氧化损伤起到保护作用。
