Department of Dermatology, University Hospital Zealand, Roskilde, Denmark.
Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Exp Dermatol. 2019 Mar;28(3):312-316. doi: 10.1111/exd.13885.
The role of bacterial biofilm in hidradenitis suppurativa (HS) is highly debated. Less biofilm is found in clinically unaffected axillary perilesional skin of HS patients compared with healthy controls.
To study the correlation between biofilm and the phenotypical characterization of the preclinical inflammatory infiltrate.
An exploratory comparative study of punch biopsies from unaffected axillary HS skin compared to similarly biopsies from healthy controls underwent standard staining procedures for CD4, CD8, CD25, FoxP3 and IL17. Standard-sized inflammatory histological hotspots were identified manually. Slides were scanned into Leica Biosystems' Digital Image Hub. Number of stained cells per slide and hotspot was found using an algorithm.
12.5% of HS had biofilm compared to 85% of controls. For full slides, HS patients had more CD4+ cells than controls; HS patients with biofilm had higher CD4+ cell number than controls with or without biofilm and HS patients without biofilm. For hotspots, HS patients with biofilm had higher number of CD4+FoxP3+ cells than HS patients without biofilm and controls with biofilm.
The association between biofilm and the number of regulatory T cells in HS patients supports the concept of dysbiosis as a factor in the preclinical HS lesions.
细菌生物膜在化脓性汗腺炎（HS）中的作用存在很大争议。与健康对照组相比，HS 患者临床无影响的腋窝病变周围皮肤中的生物膜较少。
研究生物膜与临床前炎症浸润的表型特征之间的相关性。
对来自无影响的腋窝 HS 皮肤的活检标本与来自健康对照组的类似活检标本进行了一项探索性比较研究，这些标本接受了 CD4、CD8、CD25、FoxP3 和 IL17 的标准染色程序。手动确定标准大小的炎症组织学热点。将载玻片扫描到徕卡生物系统的 Digital Image Hub 中。使用算法找到每张幻灯片和热点的染色细胞数。
12.5%的 HS 有生物膜，而 85%的对照组有生物膜。对于全幻灯片，HS 患者的 CD4+细胞比对照组多；有生物膜的 HS 患者的 CD4+细胞数高于有或没有生物膜的 HS 患者和有生物膜的对照组患者。对于热点，有生物膜的 HS 患者的 CD4+FoxP3+细胞数高于无生物膜的 HS 患者和有生物膜的对照组患者。
HS 患者生物膜与调节性 T 细胞数量之间的关联支持了生物失调作为临床前 HS 病变的一个因素的概念。
