Dhiman Sandhya, Dubey Ramesh Chand, Maheshwari Dinesh Kumar, Kumar Sandeep
Department of Botany and Microbiology, Gurukula Kangri Vishwavidyalaya, Haridwar 249-404, Uttarakhand, India.
Can J Microbiol. 2019 May;65(5):377-386. doi: 10.1139/cjm-2018-0476. Epub 2019 Jan 18.
This study aimed to harness the benefits of sulfur-oxidizing beneficial bacteria from buffalo dung to improve crop yields of . A total of 61 bacterial isolates were screened from buffalo dung, of which 40 isolates exhibited plant-growth-promoting attributes, such as phosphate solubilization, indole-3-acetic acid production, and hydrogen cyanide production. Of these 40, four bacterial isolates, viz., BUFF12, BUFF14, BUFF23, and BUFF38, were the most potent, having plant-growth-promoting and sulfur-oxidizing properties. These four isolates produced phytase by solubilizing calcium phytate and sodium phytate. They solubilized potassium besides oxidizing the sulfur, causing an increase in soil fertility and crop production. All four isolates were nonpathogenic in nature, as demonstrated by a negative haemolysis test. According to the 16S rRNA gene sequence, the isolate BUFF14 was identified as . BUFF14 maximized seed germination with enhanced vegetative and reproductive parameters during pot and field trial studies, compared with the other isolates.
本研究旨在利用水牛粪便中硫氧化有益细菌的益处来提高作物产量。从水牛粪便中总共筛选出61株细菌分离株，其中40株分离株表现出促进植物生长的特性，如溶解磷酸盐、产生吲哚-3-乙酸和产生氰化氢。在这40株中，四株细菌分离株，即BUFF12、BUFF14、BUFF23和BUFF38，最为有效，具有促进植物生长和硫氧化特性。这四株分离株通过溶解植酸钙和植酸钠产生植酸酶。它们除了氧化硫之外还溶解钾，从而提高土壤肥力和作物产量。如溶血试验阴性所示，所有四株分离株在本质上均无致病性。根据16S rRNA基因序列，分离株BUFF14被鉴定为 。与其他分离株相比，在盆栽和田间试验研究中，BUFF14使种子发芽最大化，同时增强了营养和生殖参数。
