Suzuki H, Suzuki Y, Ohtake R, Kobori H, Hashigami Y, Shimoda S I
Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Tochigi, Japan.
Pancreas. 1988;3(6):662-7. doi: 10.1097/00006676-198812000-00006.
The pituitary protein, 7B2 has been demonstrated in the pancreas and is known to be present in very high concentrations in pancreatic endocrine tumors. To investigate whether any changes in 7B2 concentrations might be present in the pancreases affected by different types of diabetes and whether the diabetic state itself might affect pituitary and hypothalamic 7B2 concentrations, streptozotocin (STZ)-treated rats and mice with natural-onset diabetes (obese hyperglycemic, or ob/ob, mice and non-obese diabetic, or NOD, mice) were employed. A significant reduction in pancreatic 7B2 concentrations was found in STZ-treated rats. The pancreatic 7B2 concentration was significantly high in ob/ob mice (p less than 0.05, versus the concentration in their lean littermates, and the decrease observed in older NOD mice, appeared to parallel their insulin reserve. Pituitary and hypothalamic 7B2 concentrations were similar in STZ-treated and control rats. A reduction in pituitary and hypothalamic 7B2 concentrations was observed in older NOD mice (both p less than 0.01 versus respective values in younger mice). In ob/ob mice, a significant reduction was also found in hypothalamic 7B2 concentration (p less than 0.01 versus that in control mice). Gel permeation chromatography showed that 7B2 immunoreactivity comprised two molecular components, and the relative proportion in the pancreas differed from that in other tissues. In the pancreas, a smaller molecular component was predominant (elution coefficient, 0.62).(ABSTRACT TRUNCATED AT 250 WORDS)
垂体蛋白7B2已在胰腺中得到证实，并且已知在胰腺内分泌肿瘤中浓度极高。为了研究受不同类型糖尿病影响的胰腺中7B2浓度是否会发生任何变化，以及糖尿病状态本身是否会影响垂体和下丘脑的7B2浓度，研究人员使用了经链脲佐菌素（STZ）处理的大鼠以及患有自然发病型糖尿病的小鼠（肥胖高血糖小鼠，即ob/ob小鼠和非肥胖糖尿病小鼠，即NOD小鼠）。研究发现，经STZ处理的大鼠胰腺中7B2浓度显著降低。ob/ob小鼠胰腺中的7B2浓度显著升高（与它们瘦型同窝小鼠的浓度相比，p小于0.05），并且在年龄较大的NOD小鼠中观察到的降低似乎与它们的胰岛素储备情况平行。经STZ处理的大鼠与对照大鼠的垂体和下丘脑7B2浓度相似。在年龄较大的NOD小鼠中观察到垂体和下丘脑7B2浓度降低（与较年轻小鼠的相应值相比，两者p均小于0.01）。在ob/ob小鼠中，下丘脑7B2浓度也显著降低（与对照小鼠相比，p小于0.01）。凝胶渗透色谱显示，7B2免疫反应性包含两个分子成分，其在胰腺中的相对比例与其他组织不同。在胰腺中，较小的分子成分占主导（洗脱系数为0.62）。（摘要截选至250字）
