Devi Anju, Yadav Achla Bharti, Kamboj Mala, Narwal Anjali, Kumar Vinay, Singh Virender
Department of Oral Pathology, Pt. B. D Sharma University of Health Sciences, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India.
Department of Conservative Dentistry, Pt. B. D Sharma University of Health Sciences, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India.
J Exp Ther Oncol. 2019 Jan;13(1):1-7.
The histogenesis process of pleomorphic adenoma (PA) of salivary gland continues to remain a controversial subject. In this neoplasm, transition occurs from an epithelial or myoepithelial cell to a stromal element and is called an epithelial-mesenchymal transition (EMT) resulting in heterogeneity. The present research was aimed to study the morphological characteristics of varied components and epithelial-mesenchymal transitions in PA using histopathological and immunohistochemical (IHC) analysis.
MATERIALS & METHODS: For this purpose 15 PAs cases were selected from the archives and from each case of PA and 5 cases of normal salivary gland, five additional sections were cut from each for immunohistochemical analysis using E- cadherin, vimentin and a-actin. The numbers of immunoreactive cells were semi- quantitatively measured and also determined the intensity of the immunostaining reaction.
Immunohistochemically, as the cells transitioned from the lumial cells to descrete/detached cells of ductal structures, the percentage of positive cells tends to decrease for E- cadherin and increases for vimentin and actin. Additionally, neoplastic cells towards the periphery of ducal-cystic structures or completely detached cells in the stroma showed intense to moderate positivity for vimentin and alpha smooth muscle actin (a-SMA). Chondroid/ hyalinized or myxoid stroma showed variable staining with these markers.
Our study provides the evidence that epithelial-mesenchymal transition process represent the basic principle of the tisuse heterogeneity in pleomorphic adenomas.
涎腺多形性腺瘤（PA）的组织发生过程一直是一个有争议的话题。在这种肿瘤中，上皮或肌上皮细胞会转变为间质成分，这一过程被称为上皮-间质转化（EMT），从而导致肿瘤的异质性。本研究旨在通过组织病理学和免疫组织化学（IHC）分析，研究PA中不同成分的形态学特征以及上皮-间质转化情况。
为此，从存档病例中选取了15例PA病例，从每例PA病例以及5例正常涎腺组织中，各额外切取5张切片用于免疫组织化学分析，检测E-钙黏蛋白、波形蛋白和α-肌动蛋白。对免疫反应阳性细胞的数量进行半定量测量，并确定免疫染色反应的强度。
免疫组织化学结果显示，随着细胞从管腔细胞转变为导管结构中的离散/分离细胞，E-钙黏蛋白阳性细胞的百分比趋于下降，波形蛋白和肌动蛋白阳性细胞的百分比则上升。此外，导管-囊性结构周边的肿瘤细胞或间质中完全分离的细胞，波形蛋白和α-平滑肌肌动蛋白（α-SMA）呈强阳性至中度阳性。软骨样/玻璃样变性或黏液样间质对这些标志物的染色情况各不相同。
我们的研究提供了证据，表明上皮-间质转化过程是多形性腺瘤组织异质性的基本原理。
