State Key Laboratory of Food Science and Technology, Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China; Jiangsu Cooperative Innovation Center of Water Treatment Technology and Materials, Suzhou University of Science and Technology, Suzhou 215009, China.
Ecotoxicol Environ Saf. 2019 Apr 30;171:683-690. doi: 10.1016/j.ecoenv.2019.01.035. Epub 2019 Jan 16.
Ozone layer depletion leads to elevated ultraviolet-B (UV-B) radiation, which affects plant growth; however, little is known about the relationship between root growth and signaling molecules in roots. Therefore, in this work, simulated UV-B radiation was used to study the effects of elevated UV-B radiation on root growth of soybean seedlings and changes in the content of signaling molecules in roots. The results showed that compared with the control, the 2.63 kJ m d and 6.17 kJ m d elevated UV-B radiation treatments inhibited root growth, and root growth parameters (total root length, root surface area, root volume, average diameter, root tip number, and root dry weight) all decreased. For root signaling molecules, the content of nitric oxide, reactive oxygen species, abscisic acid, salicylic acid, and jasmonic acid increased, and the content of auxin, cytokinin, and gibberellin decreased. The above indices changed more significantly under the 6.17 kJ m d treatment. After withdrawal of the exposure, the above indices could be restored to a certain extent. These data indicated that UV-B radiation interfered with root growth by affecting the content of signaling molecules in roots, and the degree of the effects was related to the intensity of UV-B radiation. The results from this study provide a theoretical basis for studying the preliminary mechanism of elevated UV-B radiation on root growth and possible pathways that can mitigate UV-B radiation damage for root growth. ONE SENTENCE SUMMARY: The effects of elevated UV-B on root growth of soybean seedlings were regulated by signaling molecules, and the degree of the effects was related to the intensity of UV-B radiation.
臭氧消耗导致紫外线-B（UV-B）辐射增加，从而影响植物生长；然而，人们对于根生长与根中信号分子之间的关系知之甚少。因此，在这项工作中，使用模拟的 UV-B 辐射来研究升高的 UV-B 辐射对大豆幼苗根生长的影响以及根中信号分子含量的变化。结果表明，与对照相比，2.63 kJ m d 和 6.17 kJ m d 的升高的 UV-B 辐射处理抑制了根生长，并且根生长参数（总根长、根表面积、根体积、平均直径、根尖数和根干重）均降低。对于根信号分子，一氧化氮、活性氧、脱落酸、水杨酸和茉莉酸的含量增加，而生长素、细胞分裂素和赤霉素的含量减少。在 6.17 kJ m d 处理下，上述指标变化更为明显。暴露停止后，上述指标可以在一定程度上恢复。这些数据表明，UV-B 辐射通过影响根中信号分子的含量来干扰根生长，而影响的程度与 UV-B 辐射的强度有关。本研究的结果为研究升高的 UV-B 辐射对根生长的初步机制以及可能减轻根生长中 UV-B 辐射损伤的途径提供了理论依据。
