Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Infect Disord Drug Targets. 2020;20(4):517-522. doi: 10.2174/1871526519666190119112542.
Bacteria require iron ions to grow and infect the host, which, by using iron uptake systems, acquire free iron from their host cell. Escherichia coli is one of the most important pathogens to cause food poisoning and clinical infections. The aim of this study was to assess the distribution of iron uptake systems encoding genes in clinical isolates of E.coli compared to food samples isolates.
This investigation was conducted to determine the prevalence of E. coli isolated from various sources of food and clinical specimens. The E. coli isolates confirmed by the standard microbiological methods. The isolates were examined for the presence of iut A and iuc A genes by specific primers using the polymerase chain reaction technique.
A total of 100 and 50 isolates of E. coli were collected from clinical samples and foodstuffs, respectively. The prevalence of E. coli in the food and clinical samples was 33.33% and 64.10%, respectively. The frequency of iut A and iuc A genes in the food and clinical isolates were 76%-84% and 86% - 83%, respectively.
Our results showed that the prevalence of E. coli isolates with iut A and iuc A genes was relatively higher compared to many previous studies. The existence of these genes in E. coli strains is likely to be related to pathogenicity in those strains, which requires further studies in the future.
细菌在生长和感染宿主时需要铁离子，它们通过使用铁摄取系统从宿主细胞中获取游离铁。大肠杆菌是引起食物中毒和临床感染的最重要病原体之一。本研究旨在评估与食物样本分离株相比，临床分离株大肠杆菌中铁摄取系统编码基因的分布。
本研究旨在确定从各种食物来源和临床标本中分离出的大肠杆菌的流行情况。采用标准微生物学方法确认大肠杆菌分离株。使用聚合酶链反应技术，用特异性引物检测 iut A 和 iuc A 基因的存在。
共从临床样本和食品中收集了 100 和 50 株大肠杆菌。食品和临床样本中大肠杆菌的流行率分别为 33.33%和 64.10%。食品和临床分离株中 iut A 和 iuc A 基因的频率分别为 76%-84%和 86%-83%。
我们的研究结果表明，与许多以前的研究相比，带有 iut A 和 iuc A 基因的大肠杆菌分离株的流行率相对较高。这些基因在大肠杆菌菌株中的存在可能与其在这些菌株中的致病性有关，这需要在未来进行进一步的研究。
