鉴定一种在体外产生视黄酸的蓝细菌醛脱氢酶。

Identification of a cyanobacterial aldehyde dehydrogenase that produces retinoic acid in vitro.

机构信息

School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK; Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.

School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.

出版信息

Biochem Biophys Res Commun. 2019 Feb 26;510(1):27-34. doi: 10.1016/j.bbrc.2018.12.171. Epub 2019 Jan 17.

DOI:10.1016/j.bbrc.2018.12.171

PMID:30660368

Abstract

Retinoic acid signalling is generally considered to be of animal origin. Recently, retinoic acid has been identified in cyanobacteria, yet no mechanism for its production has been identified. Here, we characterise for the first time a cyanobacterial aldehyde dehydrogenase that produces retinoic acid in vitro. Our computational studies suggest that the cyanobacterial aldehyde dehydrogenase resembles an ancestor of both eukaryotic aldehyde dehydrogenase 1 and aldehyde dehydrogenase 2. The Chlorogloeopsis fritschii aldehyde dehydrogenase described here may find applications in synthetic production of retinoic acid as well as contributing to our understanding of retinoid synthesis in cyanobacteria.

摘要

视黄酸信号通常被认为是动物起源的。最近，视黄酸已在蓝细菌中被鉴定出来，但尚未确定其产生的机制。在这里，我们首次描述了一种能够在体外产生视黄酸的蓝细菌醛脱氢酶。我们的计算研究表明，蓝细菌醛脱氢酶类似于真核醛脱氢酶 1 和醛脱氢酶 2 的共同祖先。这里描述的 Chlorogloeopsis fritschii 醛脱氢酶可能在视黄酸的合成生产中得到应用，并有助于我们理解蓝细菌中类视黄醇的合成。