Zhang Ning, Li Guohua, Li Xiaoxing, Xu Lixia, Chen Minhu
Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University Guangzhou 510080, China.
Department of Gastroenterology, Shunde People's Hospital, Nanfang Medical University Foshan 528300, China.
Am J Transl Res. 2018 Nov 15;10(11):3493-3503. eCollection 2018.
This study aimed to investigate the role of circRNAs encoded by in regulating the pathogenesis processes of hepatocellular carcinoma (HCC). Comprehensive analysis of 3 circular RNA databases revealed multiple circular RNAs within the gene. The candidate circRNAs were first structurally validated via specific convergent and divergent primer amplification, RNase R treatment, and Sanger sequencing. According to a further validation of the cell viability assay, cell cycle and apoptosis, and transwell assays, the circRNAs correlated to were obtained. Their functions in tumorigenesis were further validated via the subcutaneous tumor model and the migration model in nude mice. We showed that the overexpression of circ5379-6 decreased cell proliferation, inhibited cell migration and invasion, and induced cell apoptosis in the HCC cell lines. Consistently, studies in nude mice confirmed that the overexpression of circ5379-6 effectively inhibited the tumorigenesis and metastasis of HCC. We conclude that circ5379-6 plays a role similar to its linear counterpart to inhibit HCC tumorigenesis and progression.
本研究旨在探讨由 编码的环状RNA在调节肝细胞癌（HCC）发病机制过程中的作用。对3个环状RNA数据库的综合分析揭示了 基因内的多个环状RNA。候选环状RNA首先通过特异性的收敛和发散引物扩增、RNase R处理和Sanger测序进行结构验证。根据细胞活力测定、细胞周期和凋亡以及transwell测定的进一步验证，获得了与 相关的环状RNA。通过皮下肿瘤模型和裸鼠迁移模型进一步验证了它们在肿瘤发生中的功能。我们发现circ5379-6的过表达降低了HCC细胞系中的细胞增殖，抑制了细胞迁移和侵袭，并诱导了细胞凋亡。同样，裸鼠研究证实circ5379-6的过表达有效地抑制了HCC的肿瘤发生和转移。我们得出结论，circ5379-6发挥着与其线性对应物 类似的作用，以抑制HCC的肿瘤发生和进展。
