Oliveira Gabriela Capriogli, de Souza Almeida Henrique Meiroz, Sartori Raissa Saran, Rossi Gabriel Augusto Marques, de Oliveira Luis Guilherme, Langoni Helio
Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade Estadual Paulista (Unesp), Câmpus de Botucatu, Distrito de Rubião Junior s/n, Botucatu, SP 18618-970, Brazil.
Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista - UNESP, Câmpus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane s/n, São Paulo 14884-900, Brazil.
Parasite Epidemiol Control. 2018 Dec 12;4:e00080. doi: 10.1016/j.parepi.2018.e00080. eCollection 2019 Feb.
Toxoplasmosis is a zoonosis present worldwide. Its protozoal aethiological agent, has the ability to infect several homeothermic animals and mainly human beings. The consumption of raw or undercooked meat products containing cysts, consumption of vegetables without washing and using non-treated water are risk factors associated to the occurrence of human toxoplasmosis. Furthermore, raw or undercooked pork is an important infection source of to humans. Due to the importance of toxoplamosis in public health, this study focused on establish the prevalence of the disease in non-technified swine herds in the northeastern region of the State of São Paulo, Brazil, using Modified Agglutination Test (MAT) and the Indirect Immunofluorescence Assay (IFA) and the risk factors for its occurrence. In addition, the agreement among both diagnostic tests was evaluated. A low prevalence of toxoplasmosis was found at animal level (7.02%). The Fisher's exact test detected correlation between positive cases with the presence of food garden in the farm ( = 0.01) and the use of non-treated water to irrigate the food garden ( = 0.005. The agreement among tests was considered moderate ( = 0.5). The results show that toxoplasmosis is a risk for humans who consume under cooked pork meat in this region and an acceptable agreement between MAT and IFA tests.
弓形虫病是一种全球范围内存在的人畜共患病。其原生动物病原体能够感染多种恒温动物，主要是人类。食用含有包囊的生肉或未煮熟的肉制品、食用未清洗的蔬菜以及使用未经处理的水是与人类弓形虫病发生相关的危险因素。此外，生的或未煮熟的猪肉是人类重要的感染源。由于弓形虫病在公共卫生方面的重要性，本研究聚焦于利用改良凝集试验（MAT）和间接免疫荧光试验（IFA）确定巴西圣保罗州东北部非专业化猪群中该病的流行情况及其发生的危险因素。此外，还评估了两种诊断试验之间的一致性。在动物层面发现弓形虫病的患病率较低（7.02%）。费舍尔精确检验检测到阳性病例与农场中存在菜园（P = 0.01）以及使用未经处理的水灌溉菜园（P = 0.005）之间存在相关性。试验之间的一致性被认为是中等（κ = 0.5）。结果表明，弓形虫病对该地区食用未煮熟猪肉的人构成风险，并且MAT和IFA试验之间存在可接受的一致性。
