Nastasi A, Villafrate M R, Mammina C, Massenti M F, Caroli G, Levré E
Istituto di Igiene G. D'Alessandro, Università degli Studi di Palermo.
Boll Ist Sieroter Milan. 1988;67(1):43-8.
A molecular epidemiological analysis was carried out on S. enteritidis isolates identified at the Central Italy Enterobacteriaceae Center during 1986. 26 of these were from a diarrhoeal disease outbreak, which occurred in the period April-June at the "S. Chiara" Hospital, Pisa. All S. enteritidis strains harboured a virulence-encoding 39 MDa plasmid. The nosocomial isolates made in June 1986 carried an additional non-conjugative plasmid of 75 MDa, associated with the streptomycin-resistance. In contrast, the nosocomial S. enteritidis strains isolated during the period April-May and 16 isolates from the same geographic area were susceptible to antibiotics and showed a different plasmid pattern. According to the plasmid profile and antibiotic resistance pattern analysis, it could be argued that the hospital cases of S. enteritidis infection occurring after May 1986 are attributable to a strain having different origin from the strain circulating in April-May 1986.
对1986年在意大利中部肠杆菌科中心鉴定出的肠炎沙门氏菌分离株进行了分子流行病学分析。其中26株来自1986年4月至6月期间在比萨的“圣基亚拉”医院发生的一次腹泻病暴发。所有肠炎沙门氏菌菌株都携带一个编码毒力的39 MDa质粒。1986年6月分离出的医院菌株携带一个额外的75 MDa非接合质粒，与链霉素抗性相关。相比之下，4月至5月期间分离出的医院肠炎沙门氏菌菌株以及来自同一地理区域的16株分离株对抗生素敏感，且显示出不同的质粒图谱。根据质粒图谱和抗生素抗性模式分析，可以认为1986年5月后发生的医院肠炎沙门氏菌感染病例归因于一种与1986年4月至5月间传播的菌株来源不同的菌株。
