Department of Dermatology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Department of Dermatology, Qingdao Municipal Hospital, Qingdao, Shandong, China.
Exp Dermatol. 2019 Mar;28(3):283-291. doi: 10.1111/exd.13888.
Psoriasis is a chronic inflammatory disease, and microRNAs have been reported to regulate the pathogenesis of psoriasis. Up-regulated miR-744-3p level was identified to associate with psoriasis while the precise functions of miR-744-3p in psoriasis were not well-elucidated. We first confirmed the up-regulation of miR-744-3p in psoriasis by measuring its expression level in psoriatic samples. We explored the roles of miR-744-3p on keratinocytes proliferation and differentiation. We searched the targets of miR-744-3p and evaluated the roles of one target, KLLN on keratinocytes proliferation and differentiation. We confirmed the up-regulation of miR-744-3p in psoriatic samples. MiR-744-3p promoted keratinocytes proliferation while inhibited differentiation. MiR-744-3p targeted KLLN and overexpression of miR-744-3p resulted in decreased expression of KLLN. Overexpression of KLLN prevented the effects of miR-744-3p on keratinocytes proliferation and differentiation. MiR-744-3p regulated the proliferation and differentiation of keratinocytes through targeting KLLN in psoriasis.
银屑病是一种慢性炎症性疾病，已有研究报道 microRNAs 可调节银屑病的发病机制。上调的 miR-744-3p 水平与银屑病相关，但 miR-744-3p 在银屑病中的确切功能尚未阐明。我们首先通过测量银屑病样本中的表达水平来证实 miR-744-3p 的上调。我们探讨了 miR-744-3p 对角质形成细胞增殖和分化的作用。我们搜索了 miR-744-3p 的靶标，并评估了一个靶标 KLLN 在角质形成细胞增殖和分化中的作用。我们证实了 miR-744-3p 在银屑病样本中的上调。miR-744-3p 促进角质形成细胞增殖，同时抑制分化。miR-744-3p 靶向 KLLN，过表达 miR-744-3p 导致 KLLN 表达下调。过表达 KLLN 可阻止 miR-744-3p 对角质形成细胞增殖和分化的影响。miR-744-3p 通过靶向 KLLN 调节银屑病角质形成细胞的增殖和分化。
