一种基因编码的近红外荧光钙离子指示剂。
A genetically encoded near-infrared fluorescent calcium ion indicator.
机构信息
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
Media Lab and McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
出版信息
Nat Methods. 2019 Feb;16(2):171-174. doi: 10.1038/s41592-018-0294-6. Epub 2019 Jan 21.
We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca imaging in combination with other optogenetic indicators and actuators.
我们报告了一种强度测量、近红外荧光、基因编码钙离子（Ca）指示剂（GECI），其激发和发射最大值分别为 678nm 和 704nm。这种 GECI，被命名为 NIR-GECO1，能够对培养的哺乳动物细胞和脑组织中的 Ca 瞬变进行成像，其灵敏度可与目前可用的可见波长 GECIs 相媲美。我们证明，NIR-GECO1 与其他光遗传学指示剂和执行器结合使用，为多色 Ca 成像开辟了新的前景。
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