Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
Media Lab and McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
Nat Methods. 2019 Feb;16(2):171-174. doi: 10.1038/s41592-018-0294-6. Epub 2019 Jan 21.
We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca imaging in combination with other optogenetic indicators and actuators.
我们报告了一种强度测量、近红外荧光、基因编码钙离子（Ca）指示剂（GECI），其激发和发射最大值分别为 678nm 和 704nm。这种 GECI，被命名为 NIR-GECO1，能够对培养的哺乳动物细胞和脑组织中的 Ca 瞬变进行成像，其灵敏度可与目前可用的可见波长 GECIs 相媲美。我们证明，NIR-GECO1 与其他光遗传学指示剂和执行器结合使用，为多色 Ca 成像开辟了新的前景。
