Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT), Vellore, Tamilnadu, India.
Bionivid Technology Private Limited, Bengaluru, India.
Nanomedicine. 2019 Apr;17:94-105. doi: 10.1016/j.nano.2019.01.003. Epub 2019 Jan 19.
MicroRNAs (miRNAs) are noncoding small RNA molecules that act as decisive roles in cell proliferation and differentiation processes by targeted inhibition of mature mRNA. In this study, miRNAs that are involved in the differentiation of neural stem cells (NSC) from human mesenchymal stem cells (MSC) were completely profiled and identified to elucidate the significant miRNAs responsible for NSC differentiation. Human MSCs were induced with NSC-differentiation cocktail containing epidermal growth factor and fibroblast growth factor under serum-free conditions. The profiling of miRNAs was done using Next-Generation sequencing system. The significant miRNAs that might be involved in the differentiation process were screened. The expression levels of target genes (ARID1A and DUSP16) of miR-561-5p and miR-138-5p were determined using western blot & quantitative PCR respectively. The results could help in developing new strategies towards optimizing the in vitro differentiation of NSCs for potential use in future clinical applications.
微小 RNA（miRNA）是一类非编码的小分子 RNA，通过靶向抑制成熟 mRNA，在细胞增殖和分化过程中发挥决定性作用。在这项研究中，我们对参与人骨髓间充质干细胞（MSC）向神经干细胞（NSC）分化的 miRNA 进行了全面分析和鉴定，以阐明负责 NSC 分化的重要 miRNA。在无血清条件下，用人 NSC 分化鸡尾酒（含表皮生长因子和成纤维细胞生长因子）诱导 MSC。使用下一代测序系统进行 miRNA 分析。筛选可能参与分化过程的显著 miRNA。使用 Western blot 和定量 PCR 分别测定 miR-561-5p 和 miR-138-5p 的靶基因（ARID1A 和 DUSP16）的表达水平。这些结果有助于制定新的策略，优化 NSCs 的体外分化，为未来的临床应用提供潜在用途。
