日本北海道梅花鹿（Cervus nippon）泰勒虫属的鉴别诊断及分子特征分析

Differential diagnosis and molecular characterization of Theileria spp. in sika deer (Cervus nippon) in Hokkaido, Japan.

作者信息

Lee Seung-Hun, Moumouni Paul Franck Adjou, Galon Eloiza May, Vudriko Patrick, Liu Mingming, Benedicto Byamukama, Tumwebaze Maria Agnes, Boldbaatar Damdinsuren, Umemiya-Shirafuji Rika, Fukumoto Shinya, Xuan Xuenan

机构信息

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.

Institute of Veterinary Medicine, Zaisan 17024, Ulaanbaatar, Mongolia.

出版信息

Parasitol Int. 2019 Jun;70:23-26. doi: 10.1016/j.parint.2019.01.005. Epub 2019 Jan 18.

DOI:10.1016/j.parint.2019.01.005

PMID:30664981

Abstract

Sika deer (Cervus nippon) is widely distributed in Asian countries and is one of the most common wildlife animals in Hokkaido, Japan. Previous studies identified Theileria spp. in sika deer in Japan including Theileria sp. Thrivae belonging to T. cervi group and Theileria sp. sola belonging to T. capreoli group. However, the studies failed to differentiate these two species without sequencing. Therefore, epidemiological information on cervine theileriosis in Hokkaido, Japan is limited. This study differentiated the two Theileria spp. using restriction fragments length polymorphism (RFLP). Based on the PCR-RFLP, Theileria spp. were identified in 103 (88.0%) of 117 samples, and the prevalence of each parasites were 86.3% (n = 101) and 57.3% (n = 67) for Theileria sp. Thrivae and T. capreoli-like, respectively. Phylogenetic analysis based on the 18S rRNA showed a close relationship between Theileria sp. Thrivae and T. cervi in China. In addition, phylogenetic analysis of internal transcribed spacer regions also showed a close relationship between Theileria sp. Thrivae and T. cervi.

摘要

梅花鹿（Cervus nippon）广泛分布于亚洲国家，是日本北海道最常见的野生动物之一。先前的研究在日本梅花鹿体内鉴定出泰勒虫属（Theileria spp.），包括属于鹿泰勒虫（T. cervi）组的泰勒虫属Thrivae种和属于羊泰勒虫（T. capreoli）组的泰勒虫属sola种。然而，这些研究在未进行测序的情况下无法区分这两个物种。因此，关于日本北海道鹿泰勒虫病的流行病学信息有限。本研究利用限制性片段长度多态性（RFLP）区分了这两种泰勒虫属物种。基于PCR-RFLP，在117个样本中的103个（88.0%）中鉴定出泰勒虫属物种，泰勒虫属Thrivae种和类羊泰勒虫的各寄生虫感染率分别为86.3%（n = 101）和57.3%（n = 67）。基于18S rRNA的系统发育分析表明，泰勒虫属Thrivae种与中国的鹿泰勒虫关系密切。此外，内部转录间隔区的系统发育分析也表明泰勒虫属Thrivae种与鹿泰勒虫关系密切。