通过流式细胞术评估抗体依赖性细胞介导的细胞毒性

Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry.

作者信息

van der Haar Àvila Irene, Marmol Patricia, Cany Jeannette, Kiessling Rolf, Pico de Coaña Yago

机构信息

Department of Oncology-Pathology, Karolinska Institute, Stockholm, Sweden.

Division of Tumor Biology and Immunology (B3), NKI-AvL, Amsterdam, The Netherlands.

出版信息

Methods Mol Biol. 2019;1913:181-194. doi: 10.1007/978-1-4939-8979-9_13.

DOI:10.1007/978-1-4939-8979-9_13

PMID:30666607

Abstract

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism in which immune cell activation is induced by the cross-linking of CD16 with the Fc region of antibodies that at the same time bind specifically to cell surface antigens. ADCC stimulates the secretion of perforin, granzymes, and cytokines leading to lysis of the malignant cells. Natural killer (NK) cells express the CD16 receptor and can therefore be activated by ADCC to kill tumor cells. To study the cytotoxicity of NK cells against cancer cells, an ADCC-based assay is described: the flow cytometry-based cytotoxicity assay. In this method, the antibody trastuzumab, which binds specifically to HER2-positive malignant cells, is used to trigger ADCC.

摘要

抗体依赖性细胞介导的细胞毒性作用（ADCC）是一种机制，在此机制中，CD16与同时特异性结合细胞表面抗原的抗体的Fc区域交联可诱导免疫细胞活化。ADCC刺激穿孔素、颗粒酶和细胞因子的分泌，导致恶性细胞裂解。自然杀伤（NK）细胞表达CD16受体，因此可被ADCC激活以杀伤肿瘤细胞。为研究NK细胞对癌细胞的细胞毒性，描述了一种基于ADCC的检测方法：基于流式细胞术的细胞毒性检测。在该方法中，特异性结合HER2阳性恶性细胞的抗体曲妥珠单抗用于触发ADCC。