环境、健康史、T 区淋巴瘤及意义未明的 T 区样细胞之间的关联：一项针对老年金毛猎犬的病例对照研究。
Associations of environment, health history, T-zone lymphoma, and T-zone-like cells of undetermined significance: A case-control study of aged Golden Retrievers.
作者信息
Labadie Julia D, Magzamen Sheryl, Morley Paul S, Anderson G Brooke, Yoshimoto Janna, Avery Anne C
机构信息
Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado.
Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado.
出版信息
J Vet Intern Med. 2019 Mar;33(2):764-775. doi: 10.1111/jvim.15405. Epub 2019 Jan 21.
BACKGROUND
T-zone lymphoma (TZL), an indolent disease in older dogs, comprises approximately 12% of lymphomas in dogs. TZL cells exhibit an activated phenotype, indicating the disease may be antigen-driven. Prior research found that asymptomatic aged Golden Retrievers (GLDRs) commonly have populations of T-zone-like cells (phenotypically identical to TZL) of undetermined significance (TZUS).
OBJECTIVE
To evaluate associations of inflammatory conditions, TZL and TZUS, using a case-control study of GLDRs.
ANIMALS
TZL cases (n = 140), flow cytometrically diagnosed, were identified through Colorado State University's Clinical Immunology Laboratory. Non-TZL dogs, recruited through either a database of owners interested in research participation or the submitting clinics of TZL cases, were subsequently flow cytometrically classified as TZUS (n = 221) or control (n = 147).
METHODS
Health history, signalment, environmental, and lifestyle factors were obtained from owner-completed questionnaires. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were estimated using multivariable logistic regression, obtaining separate estimates for TZL and TZUS (versus controls).
RESULTS
Hypothyroidism (OR, 0.3; 95% CI, 0.1-0.7), omega-3 supplementation (OR, 0.3; 95% CI, 0.1-0.6), and mange (OR, 5.5; 95% CI, 1.4-21.1) were significantly associated with TZL. Gastrointestinal disease (OR, 2.4; 95% CI, 0.98-5.8) had nonsignificantly increased TZL odds. Two shared associations for TZL and TZUS were identified: bladder infection or calculi (TZL OR, 3.5; 95% CI, 0.96-12.7; TZUS OR, 5.1; 95% CI, 1.9-13.7) and eye disease (TZL OR, 2.3; 95% CI, 0.97-5.2; TZUS OR, 1.9; 95% CI, 0.99-3.8).
CONCLUSIONS AND CLINICAL IMPORTANCE
These findings may elucidate pathways involved in TZUS risk and progression from TZUS to TZL. Further investigation into the protective association of omega-3 supplements is warranted.
背景
T 区淋巴瘤（TZL）是老年犬的一种惰性疾病，约占犬淋巴瘤的 12%。TZL 细胞表现出活化表型，表明该疾病可能由抗原驱动。先前的研究发现，无症状的老年金毛寻回犬（GLDR）通常存在意义未明的 T 区样细胞群体（表型与 TZL 相同）（TZUS）。
目的
通过对 GLDR 进行病例对照研究，评估炎症性疾病、TZL 和 TZUS 之间的关联。
动物
通过科罗拉多州立大学临床免疫学实验室鉴定出 140 例经流式细胞术诊断的 TZL 病例。通过对有研究参与意愿的犬主数据库或 TZL 病例的提交诊所招募的非 TZL 犬，随后经流式细胞术分类为 TZUS（221 例）或对照（147 例）。
方法
从犬主填写的问卷中获取健康史、特征、环境和生活方式因素。使用多变量逻辑回归估计比值比（OR）和 95%置信区间（95%CI），分别获得 TZL 和 TZUS（与对照相比）的估计值。
结果
甲状腺功能减退（OR，0.3；95%CI，0.l - 0.7）、补充ω-3（OR，0.3；95%CI，0.l - 0.6）和疥螨病（OR，5.5；95%CI，1.4 - 21.1）与 TZL 显著相关。胃肠道疾病（OR，2.4；95%CI，0.98 - 5.8）使 TZL 的患病几率有非显著增加。确定了 TZL 和 TZUS 的两个共同关联因素：膀胱感染或结石（TZL 的 OR，3.5；95%CI，0.96 - 12.7；TZUS 的 OR，5.1；95%CI，1.9 - 13.7）和眼部疾病（TZL 的 OR，2.3；95%CI，0.97 - 5.2；TZUS 的 OR，1.9；95%CI，0.99 - 3.8）。
结论及临床意义
这些发现可能阐明 TZUS 风险以及从 TZUS 进展为 TZL 所涉及的途径。有必要进一步研究ω-3 补充剂具有的保护关联。
Zotero 插件