Seelig D M, Avery P, Webb T, Yoshimoto J, Bromberek J, Ehrhart E J, Avery A C
Veterinary Clinical Sciences, University of Minnesota, St. Paul, MN.
J Vet Intern Med. 2014 May-Jun;28(3):878-86. doi: 10.1111/jvim.12343. Epub 2014 Mar 21.
Canine T-cell lymphoma (TCL) is clinically and histologically heterogeneous with some forms, such as T-zone lymphoma (TZL), having an indolent course. Immunophenotyping is an important tool in the classification of TCL in people, and can be equally useful in dogs.
HYPOTHESIS/OBJECTIVES: We hypothesized that loss of expression of the CD45 antigen is a specific diagnostic feature of TZL.
Twenty dogs with concurrent histology and immunophenotyping by flow cytometry were studied in depth. An additional 494 dogs diagnosed by immunophenotyping were used to characterize the population of dogs with this disease.
Lymph node biopsies from 35 dogs with TCL were classified by 2 pathologists using WHO criteria. Twenty lymph nodes were from dogs with CD45- TCL and 15 were from CD45+ TCL. The pathologists were blinded to the flow cytometry findings. Outcome information was sought for the 20 dogs with CD45- lymphoma, and population characteristics of the additional 494 dogs were described.
All 20 CD45- cases were classified as TZL. The 15 CD45+ cases were classified as aggressive TCL and are described in an accompanying paper. TZL cases had a median survival of 637 days. Examination of 494 additional dogs diagnosed with TZL by immunophenotyping demonstrated that 40% of cases are in Golden Retrievers, are diagnosed at a median age of 10 years, and the majority have lymphadenopathy and lymphocytosis.
TZL has unique immunophenotypic features that can be used for diagnosis.
犬T细胞淋巴瘤（TCL）在临床和组织学上具有异质性，某些类型，如T细胞区淋巴瘤（TZL），病程较为惰性。免疫表型分析是人类TCL分类中的重要工具，在犬类中同样有用。
假设/目的：我们假设CD45抗原表达缺失是TZL的特异性诊断特征。
对20只同时进行组织学检查和流式细胞术免疫表型分析的犬进行了深入研究。另外494只经免疫表型分析诊断的犬用于描述患有这种疾病的犬群特征。
35只患有TCL的犬的淋巴结活检标本由2名病理学家根据世界卫生组织标准进行分类。20个淋巴结来自患有CD45 - TCL的犬，15个来自CD45 + TCL的犬。病理学家对流式细胞术结果不知情。对20只患有CD45 - 淋巴瘤的犬寻求预后信息，并描述另外494只犬的群体特征。
所有20例CD45 - 病例均被分类为TZL。15例CD45 + 病例被分类为侵袭性TCL，相关内容在一篇配套论文中描述。TZL病例的中位生存期为637天。对另外494只经免疫表型分析诊断为TZL的犬进行检查发现，40%的病例为金毛寻回犬，中位诊断年龄为10岁，大多数有淋巴结病和淋巴细胞增多症。
TZL具有可用于诊断的独特免疫表型特征。
