小鼠胎儿和新生生殖细胞中的性别特异性组蛋白修饰。
Sex-specific histone modifications in mouse fetal and neonatal germ cells.
机构信息
Department of Bioscience, Tokyo University of Agriculture, Setagaya, Tokyo, Japan.
NODAI Genome Research Centre, Tokyo University of Agriculture, Setagaya, Tokyo, Japan.
出版信息
Epigenomics. 2019 Apr;11(5):543-561. doi: 10.2217/epi-2018-0193. Epub 2019 Jan 22.
AIMS
Epigenetic signatures of germline cells are dynamically reprogrammed to induce appropriate differentiation, development and sex specification. We investigated sex-specific epigenetic changes in mouse fetal germ cells (FGCs) and neonatal germ cells.
MATERIALS & METHODS: Six histone marks in mouse E13.5 FGCs and P1 neonatal germ cells were analyzed by chromatin immunoprecipitation and sequencing. These datasets were compared with transposase-accessible chromatin sites, DNA methylation and transcriptome.
RESULTS
Different patterns of each histone mark were detected in female and male FGCs, and H3K4me3/H3K27me3 bivalent marks were enriched in different chromosomal regions of female and male FGCs.
CONCLUSION
Our results suggest that histone modifications may affect FGC gene expression following DNA methylation erasure, contributing to the differentiation into female and male germ cells.
目的
生殖细胞的表观遗传特征被动态重编程，以诱导适当的分化、发育和性别决定。我们研究了小鼠胎儿生殖细胞（FGC）和新生生殖细胞中的性别特异性表观遗传变化。
材料与方法
通过染色质免疫沉淀和测序分析了 E13.5 期 FGC 和 P1 期新生生殖细胞中的 6 种组蛋白标记。这些数据集与转座酶可及染色质位点、DNA 甲基化和转录组进行了比较。
结果
在雌性和雄性 FGC 中检测到了不同的每种组蛋白标记模式，并且 H3K4me3/H3K27me3 双价标记在雌性和雄性 FGC 的不同染色体区域富集。
结论
我们的结果表明，组蛋白修饰可能会影响 DNA 甲基化消除后的 FGC 基因表达，有助于分化为雌性和雄性生殖细胞。
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