Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), BP 74, 13 Place Pasteur, Belvédère, 1002 Tunis, Tunisia.
Laboratory of Bioinformatics, Biomathematics and Biostatistics, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunisia.
Biomed Res Int. 2018 Dec 23;2018:7943786. doi: 10.1155/2018/7943786. eCollection 2018.
The aim of the current study is to assess the prevalence of infection in broiler chickens, raised in intensive production conditions, and to evaluate the antimicrobial susceptibility of recovered isolates. A total of 590 cloacal swab samples were taken from 13 broiler chicken flocks in the North East of Tunisia. All samples were tested for the presence of thermophilic by culture and PCR, targeting the and genes, respectively. Susceptibility to antimicrobial drugs was tested against 8 antibiotics. Prevalence of infection, relationship with geographic origins and seasons, antimicrobial resistance rates and patterns were analyzed. Total prevalence of infection in broiler flocks was in the range of 22.4%, with a predominance of (68.9%), followed by (31.1%). Positive association was highlighted between the infection level and the season ( < 0.001), but no link was emphasized considering the geographic origin. Antimicrobial susceptibility testing revealed very high resistance rates detected against macrolide, tetracycline, quinolones, and chloramphenicol, ranging from 88.6% to 100%. Lower resistance prevalence was noticed for -lactams (47% and 61.4%) and gentamicin (12.9%). 17 R-type patterns were observed, and a common pattern was found in 30.3% of isolates. This study provides updates and novel data on the prevalence and the AMR of broiler campylobacters in Tunisia, revealing the occurrence of high resistance to several antibiotics and emphasizing the requirement of better surveillance and careful regulation of antimicrobials use.
本研究旨在评估在密集生产条件下饲养的肉鸡中 感染的流行情况，并评估回收分离株的抗菌药物敏感性。从突尼斯东北部的 13 个肉鸡鸡群中采集了 590 份直肠拭子样本。所有样本均通过培养和 PCR 检测耐热 的存在，分别针对 和 基因。对 8 种抗生素进行了药敏试验。分析了感染的流行率、与地理起源和季节的关系、抗药性率和模式。肉鸡群中 的总感染率在 22.4%的范围内，以 （68.9%）为主，其次是 （31.1%）。感染水平与季节之间存在显著的正相关关系（<0.001），但考虑到地理起源，并没有强调这种关联。抗菌药物敏感性试验显示，对大环内酯类、四环素类、喹诺酮类和氯霉素的耐药率非常高，范围从 88.6%到 100%。对 -内酰胺类（47%和 61.4%）和庆大霉素（12.9%）的耐药率较低。观察到 17 种 R 型模式，其中 30.3%的分离株存在共同模式。本研究提供了突尼斯肉鸡弯曲杆菌的流行率和 AMR 的最新数据和新信息，显示出对几种抗生素的高耐药性，并强调需要更好的监测和谨慎管理抗生素的使用。
