Facultad de Medicina Veterinaria y Zootecnia. Universidad Central del Ecuador, Quito, Ecuador.
Center for Molecular Diagnosis, LHUB-ULB, Brussels, Belgium.
Poult Sci. 2017 Jul 1;96(7):2366-2374. doi: 10.3382/ps/pew487.
Thermotolerant Campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. The linkage of human campylobacteriosis and poultry has been widely described. In this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of C. coli and C. jejuni in broilers from Ecuador. Caecal content from 379 randomly selected broiler batches originating from 115 farms were collected from 6 slaughterhouses located in the province of Pichincha during 1 year. Microbiological isolation was performed by direct plating on mCCDA agar. Identification of Campylobacter species was done by PCR. Minimum inhibitory concentration (MIC) values for gentamicin, ciprofloxacin, nalidixic acid, tetracycline, streptomycin, and erythromycin were obtained. Genetic variation was assessed by RFLP-flaA typing and Multilocus Sequence Typing (MLST) of selected isolates. Prevalence at batch level was 64.1%. Of the positive batches 68.7% were positive for C. coli, 18.9% for C. jejuni, and 12.4% for C. coli and C. jejuni. Resistance rates above 67% were shown for tetracycline, ciprofloxacin, and nalidixic acid. The resistance pattern tetracycline, ciprofloxin, and nalidixic acid was the dominant one in both Campylobacter species. RFLP-flaA typing analysis showed that C. coli and C. jejuni strains belonged to 38 and 26 profiles respectively. On the other hand MLST typing revealed that C. coli except one strain belonged to CC-828, while C. jejuni except 2 strains belonged to 12 assigned clonal complexes (CCs). Furthermore 4 new sequence types (STs) for both species were described, whereby 2 new STs for C. coli were based on new allele sequences. Further research is necessary to estimate the impact of the slaughter of Campylobacter positive broiler batches on the contamination level of carcasses in slaughterhouses and at retail in Ecuador.
耐热弯曲菌属是全球食源性胃肠道感染的主要原因。人类弯曲菌病和家禽之间的联系已被广泛描述。在这项研究中，我们旨在调查厄瓜多尔肉鸡中大肠杆菌和空肠弯曲菌的流行率、抗药性和遗传多样性。从皮钦查省 6 个屠宰场的 115 个农场中随机选择的 379 批肉鸡中采集盲肠内容物。通过直接在 mCCDA 琼脂上进行平板培养进行微生物分离。通过 PCR 鉴定弯曲菌属种。获得庆大霉素、环丙沙星、萘啶酸、四环素、链霉素和红霉素的最小抑菌浓度 (MIC) 值。通过 RFLP-flaA 分型和选定分离株的多位点序列分型 (MLST) 评估遗传变异。批次水平的患病率为 64.1%。阳性批次中，68.7%为大肠杆菌阳性，18.9%为空肠弯曲菌阳性，12.4%为大肠杆菌和空肠弯曲菌阳性。四环素、环丙沙星和萘啶酸的耐药率均高于 67%。在两种弯曲菌中，四环素、环丙沙星和萘啶酸的耐药模式均占主导地位。RFLP-flaA 分型分析显示，大肠杆菌和空肠弯曲菌菌株分别属于 38 和 26 种。另一方面，MLST 分型显示，除一株外，大肠杆菌均属于 CC-828，而空肠弯曲菌除 2 株外，均属于 12 个指定的克隆复合体 (CC)。此外，还描述了两种新的种间序列类型 (ST)，其中 2 种新的大肠杆菌 ST 基于新的等位基因序列。需要进一步研究来评估屠宰弯曲菌阳性肉鸡批次对厄瓜多尔屠宰场和零售环节胴体污染水平的影响。
