Department of Plant Science, University of Zambia, Lusaka, Zambia.
USDA-ARS, Sugarbeet and Bean Research Unit, Michigan State University, 1066 Bogue St, East Lansing, MI, 48824, USA.
Theor Appl Genet. 2019 May;132(5):1375-1387. doi: 10.1007/s00122-019-03284-6. Epub 2019 Jan 22.
QTL were identified for symbiotic nitrogen fixation in common bean. These QTL were detected in both greenhouse and field studies, and many overlapped with previously reported QTL in diverse mapping populations. Common bean (Phaseolus vulgaris L.) productivity can be improved through the genetic enhancement of its symbiotic nitrogen fixation (SNF) capacity. This study was aimed at understanding the genetic architecture of SNF through QTL analysis of a recombinant inbred line (RIL) population contrasting for SNF potential. The mapping population consisted of 188 F RILs derived from a cross of Solwezi and AO-1012-29-3-3A that were evaluated for SNF in the greenhouse and field in Zambia. The population was genotyped with 5398 single-nucleotide polymorphism (SNP) markers. QTL for shoot biomass, nitrogen percentage in shoot biomass, nitrogen percentage in seed, total nitrogen derived from atmosphere (Ndfa) and percentage of nitrogen derived from the atmosphere (%Ndfa) were identified. Three QTL for %Ndfa were identified on chromosomes Pv01, Pv04 and Pv09. Five QTL for Ndfa were identified on Pv04, Pv06, Pv07, Pv09 and Pv11. The QTL Ndfa9.1 identified in the current study overlapped with a previously reported QTL for SNF. A major QTL Ndfa7.1 (R = 14.9%) was consistently identified in two greenhouse studies and overlapped with previously reported QTL. The QTL Ndfa4.2 identified from the greenhouse experiment is novel and overlapped with the QTL %NB4.3, %NS4.2 and %Ndfa4.2 from the field experiment. These QTL identified in both greenhouse and field experiments, which overlap with previously reported QTL, could potentially be deployed by marker-assisted breeding to accelerate development of bean cultivars with enhanced SNF.
在普通菜豆中鉴定到了共生固氮的 QTL。这些 QTL 在温室和田间研究中均有检测到，许多与在不同作图群体中先前报道的 QTL 重叠。通过遗传增强普通菜豆（Phaseolus vulgaris L.）的共生固氮（SNF）能力，可以提高其生产力。本研究旨在通过对具有固氮潜力差异的重组自交系（RIL）群体进行 QTL 分析，了解 SNF 的遗传结构。作图群体由 Solwezi 和 AO-1012-29-3-3A 杂交衍生的 188 个 F6 RIL 组成，这些 RIL 在赞比亚的温室和田间条件下评估了 SNF 能力。该群体使用 5398 个单核苷酸多态性（SNP）标记进行了基因型分析。鉴定到了与地上部生物量、地上部氮含量百分比、种子氮含量百分比、来自大气的总氮（Ndfa）和来自大气的氮百分比（%Ndfa）相关的 QTL。在 Pv01、Pv04 和 Pv09 染色体上鉴定到了 3 个与 %Ndfa 相关的 QTL。在 Pv04、Pv06、Pv07、Pv09 和 Pv11 染色体上鉴定到了 5 个与 Ndfa 相关的 QTL。本研究中鉴定到的 Ndfa9.1 与先前报道的 SNF 相关 QTL 重叠。在两个温室研究中一致鉴定到的一个主要的 Ndfa7.1（R=14.9%）与先前报道的 QTL 重叠。从温室试验中鉴定到的 Ndfa4.2 是一个新的 QTL，与田间试验中的 QTL %NB4.3、%NS4.2 和 %Ndfa4.2 重叠。这些在温室和田间试验中鉴定到的、与先前报道的 QTL 重叠的 QTL，可能会通过标记辅助选择来加速开发具有增强 SNF 能力的菜豆品种。
