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菜豆(Phaseolus vulgaris L.)豆荚品质和产量性状的数量性状基因座定位

QTL mapping for pod quality and yield traits in snap bean ( L.).

作者信息

Njau Serah Nyawira, Parker Travis A, Duitama Jorge, Gepts Paul, Arunga Edith Esther

机构信息

Department of Water and Agricultural Resource Management, University of Embu, Embu, Kenya.

Department of Plant Sciences, University of California, Davis, Davis, CA, United States.

出版信息

Front Plant Sci. 2024 Aug 12;15:1422957. doi: 10.3389/fpls.2024.1422957. eCollection 2024.

Abstract

Pod quality and yield traits in snap bean ( L.) influence consumer preferences, crop adoption by farmers, and the ability of the product to be commercially competitive locally and globally. The objective of the study was to identify the quantitative trait loci (QTL) for pod quality and yield traits in a snap × dry bean recombinant inbred line (RIL) population. A total of 184 F RILs derived from a cross between Vanilla (snap bean) and MCM5001 (dry bean) were grown in three field sites in Kenya and one greenhouse environment in Davis, CA, USA. They were genotyped at 5,951 single nucleotide polymorphisms (SNPs), and composite interval mapping was conducted to identify QTL for 16 pod quality and yield traits, including pod wall fiber, pod string, pod size, and harvest metrics. A combined total of 44 QTL were identified in field and greenhouse trials. The QTL for pod quality were identified on chromosomes Pv01, Pv02, Pv03, Pv04, Pv06, and Pv07, and for pod yield were identified on Pv08. Co-localization of QTL was observed for pod quality and yield traits. Some identified QTL overlapped with previously mapped QTL for pod quality and yield traits, with several others identified as novel. The identified QTL can be used in future marker-assisted selection in snap bean.

摘要

菜豆的豆荚品质和产量性状会影响消费者偏好、农民对作物的接受程度以及产品在本地和全球市场上的商业竞争力。本研究的目的是在菜豆×干豆重组自交系(RIL)群体中鉴定豆荚品质和产量性状的数量性状基因座(QTL)。从香草(菜豆)和MCM5001(干豆)杂交后代中获得的总共184个F RILs在肯尼亚的三个田间地点和美国加利福尼亚州戴维斯的一个温室环境中种植。对它们进行了5951个单核苷酸多态性(SNP)的基因分型,并进行复合区间作图以鉴定16个豆荚品质和产量性状的QTL,包括豆荚壁纤维、豆荚筋、豆荚大小和收获指标。在田间和温室试验中总共鉴定出44个QTL。在Pv01、Pv02、Pv03、Pv04、Pv06和Pv07染色体上鉴定出了豆荚品质的QTL,在Pv08染色体上鉴定出了豆荚产量的QTL。观察到豆荚品质和产量性状的QTL共定位。一些鉴定出的QTL与先前定位的豆荚品质和产量性状的QTL重叠,其他几个则被鉴定为新的。所鉴定的QTL可用于未来菜豆的标记辅助选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bf4/11345156/bf21db413976/fpls-15-1422957-g001.jpg

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