Wang Qiang, Liu Zhibin, Wang Yuan, Li Jie, Lu Gang, Jing Zhenqi, Liu Yao, Guo Yang
Innovation Research Institute of Combined Acupuncture and Medicine, Shaanxi University of CM, Xianyang 712046, China; Shaanxi Key Laboratory of Combined Acupuncture and Medicine, Xianyang 712046.
Innovation Research Institute of Combined Acupuncture and Medicine, Shaanxi University of CM, Xianyang 712046, China.
Zhongguo Zhen Jiu. 2018 Oct 12;38(10):1093-7. doi: 10.13703/j.0255-2930.2018.10.016.
To observe the effects of treatment on the ultrastructure of olfactory bulb and the expression of substantia nigra glial fibrillary acidic protein (GFAP) in mice with Parkinson's disease (PD) induced by lipopolysaccharide (LPS), and to provide methods and evidence for early prevention and treatment of PD.
Forty C57BL/6 male mice were randomly divided into a blank group, a model group, an electroacupuncture (EA) group and a medication group, 10 mice in each one. The mice in the model group, EA group and medication group were treated with 30-day nasal perfusion of LPS to establish PD model. From the first day of model establishment, the mice in the EA group were treated with electroacupuncture at bilateral "Yingxiang" (LI 20) and "Yintang" (GV 29) for 20 min, once a day; 5-day treatment was taken as one session, and 4 sessions were given with an interval of 2 days between sessions. The mice in the medication group were treated with intraperitoneal injection of L-DOPA, 10 mg/mL, once a day; 5-day treatment was taken as one session, and 4 sessions were given with an interval of 2 days between sessions. After treatment, the behavioristics changes were observed by using footprint analysis and swimming test score; the ultrastructure of olfactory bulb was observed by using transmission electron microscopy; the expression of GFAP in substantia nigra was measured by using western blot method.
① After model establishment, the mice in the model group, the EA group and medication group showed significant symptoms of quiver and fear of chill, and the BMI was significantly lower than that in the blank group (all <0.05). The results of behavioristics test indicated footprint length and swimming time in the model group, the EA group and medication group was significantly lower than those in the blank group (all <0.01), indicating the successful establishment of PD model. ② After one-session treatment, the symptoms of quiver and fear of chill were not observed in the EA group. After 4-session treatment, the BMI in the EA group was significantly higher than that in the model group (<0.05); the symptoms of quiver and fear of chill were not observed in the medication group, but the BMI was similar with the model group (>0.05). ③ After treatment, the footprint and swimming time in the model group were significantly lower than that in the blank group (both <0.01); the footprint and swimming time in the EA group and medication group were significantly higher than those in the model group (all <0.01).④ After treatment, compared with the blank group, the organelles and ultrastructure of olfactory bulb in the model group were significantly improved; the ultrastructure of olfactory bulb in the EA group was improved compared with that in the model group. ⑤ After treatment, the expression of substantia nigra GFAP in the model group was significantly higher than that in the blank group (<0.01); the expression of substantia nigra GFAP in the EA group and medication group was significantly lower than that in the model group (both <0.05).
The early treatment of can improve behavioral disorders in LPS-induced early PD mice, and the mechanism may be related to the regulation of olfactory disorders and the expression of GFAP in substantia nigra.
观察治疗对脂多糖（LPS）诱导的帕金森病（PD）小鼠嗅球超微结构及黑质胶质纤维酸性蛋白（GFAP）表达的影响，为PD的早期防治提供方法和依据。
将40只C57BL/6雄性小鼠随机分为空白组、模型组、电针组和药物组，每组10只。模型组、电针组和药物组小鼠采用30天鼻腔灌注LPS建立PD模型。从造模第1天起，电针组小鼠取双侧“迎香”（LI 20）和“印堂”（GV 29）进行电针治疗20分钟，每天1次；5天治疗为1个疗程，共给予4个疗程，疗程间隔2天。药物组小鼠腹腔注射左旋多巴，10mg/mL，每天1次；5天治疗为1个疗程，共给予4个疗程，疗程间隔2天。治疗后，采用足印分析和游泳试验评分观察行为学变化；采用透射电子显微镜观察嗅球超微结构；采用蛋白质免疫印迹法检测黑质中GFAP的表达。
①造模后，模型组、电针组和药物组小鼠均出现明显的震颤和畏寒症状，且体重指数（BMI）显著低于空白组（均<0.05）。行为学测试结果显示，模型组、电针组和药物组的足印长度和游泳时间均显著低于空白组（均<0.01），表明PD模型建立成功。②1个疗程治疗后，电针组未观察到震颤和畏寒症状。4个疗程治疗后，电针组的BMI显著高于模型组（<0.05）；药物组未观察到震颤和畏寒症状，但BMI与模型组相似（>0.05）。③治疗后，模型组的足印和游泳时间显著低于空白组（均<0.01）；电针组和药物组的足印和游泳时间均显著高于模型组（均<0.01）。④治疗后，与空白组相比，模型组嗅球的细胞器和超微结构明显改善；电针组嗅球的超微结构较模型组有所改善。⑤治疗后，模型组黑质GFAP的表达显著高于空白组（<0.01）；电针组和药物组黑质GFAP的表达均显著低于模型组（均<0.05）。
早期治疗可改善LPS诱导的早期PD小鼠的行为障碍，其机制可能与调节嗅觉障碍及黑质中GFAP的表达有关。
