Cols P, Apelgot S, Guille E
Institut Curie, Section de Physique et Chimie, Paris, France.
Radiat Environ Biophys. 1988;27(4):261-75. doi: 10.1007/BF01209755.
When circular single-stranded DNA of phage S13 is labelled with 32P or 33P, the transmutations very efficiently bring about a loss of phage infectiousness (efficiency = 1 for 32P and 0.73 for 33P). For both radionuclides, the lethal efficiencies as well as the lethal events are different. In the case of 32P, the lethal event is the loss of the circular integrity of the DNA molecule, occurring as a consequence of a systematic single strand-break caused by each 32P decay (100%). Conversely, in the case of 33P, the lethal events are either a single strand-break (40%) or a local stereochemical modification (33%). The same primary event, the substitution at each 33P decay of a phosphate by a sulfate molecule, leads to one of these lethal events in relation to the decay site. Moreover, neither the phage adsorption nor its genome injection into bacteria depends on the physical state of the genome, and thus lethality is revealed at only the genetic level.
当用³²P或³³P标记噬菌体S13的环状单链DNA时，这些核转变能非常有效地导致噬菌体感染力丧失（³²P的效率为1，³³P的效率为0.73）。对于这两种放射性核素，致死效率和致死事件均有所不同。就³²P而言，致死事件是DNA分子环状完整性的丧失，这是由每次³²P衰变导致的系统性单链断裂引起的（100%）。相反，就³³P而言，致死事件要么是单链断裂（40%），要么是局部立体化学修饰（33%）。相同的初始事件，即每次³³P衰变时一个磷酸被一个硫酸分子取代，会根据衰变位点导致这些致死事件之一。此外，噬菌体吸附及其基因组注入细菌均不依赖于基因组的物理状态，因此致死性仅在基因水平上显现出来。
