The United Graduate School of Agriculture Science, Gifu University, Gifu 501-1193, Japan.
Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Plant Dis. 2018 Jul;102(7):1357-1364. doi: 10.1094/PDIS-10-17-1679-RE. Epub 2018 May 8.
Hydroponic culture systems are subject to high risks of diseases caused by zoosporic plant pathogens. Control is generally difficult because of the rapid spread of zoospores in the nutrient solutions. In Japan, tomato and eustoma, which are cultivated using the D-tray and nutrient film techniques, respectively, are susceptible to diseases caused by Pythium aphanidermatum and P. irregulare. We used loop-mediated isothermal amplification to identify potential contamination sources of these two pathogens by monitoring their presence in the water supply wells, seedling terraces, nutrient solutions, diseased plants, and ground soils of a tomato greenhouse complex and a eustoma greenhouse complex. The results indicated that the pathogens may enter the culture systems from the soils around the greenhouses. Entry most likely occurs when seedlings are moved from the seedling terraces to the greenhouses, and sterilization of the hydroponic systems may not be sufficient. Therefore, monitoring pathogens in the culture systems and ground soils is very important for the management and prevention of these diseases.
水培栽培系统容易受到游动孢子植物病原菌引起的疾病的影响。由于游动孢子在营养液中迅速传播，因此通常难以控制。在日本，使用 D 型托盘和营养液膜技术分别栽培的番茄和六出花容易受到腐霉属和不规则腐霉属引起的疾病的影响。我们使用环介导等温扩增技术来监测供水井、苗床、营养液、患病植物和番茄温室综合体及六出花温室综合体地面土壤中这些两种病原体的存在，以确定这些潜在的污染来源。结果表明，病原体可能从温室周围的土壤进入栽培系统。当幼苗从苗床移到温室时，病原体最有可能进入，并且水培系统的消毒可能不够充分。因此，监测栽培系统和地面土壤中的病原体对于这些疾病的管理和预防非常重要。
