Galla Luisa, Pizzo Paola, Greotti Elisa
Department of Biomedical Sciences, University of Padua, Padua, Italy.
Neuroscience Institute -Italian National Research Council (CNR), Padua, Italy.
Methods Mol Biol. 2019;1925:15-30. doi: 10.1007/978-1-4939-9018-4_2.
Calcium ion (Ca) is a ubiquitous intracellular messenger able to generate versatile intracellular signals that modulate a large variety of functions in virtually every cell type. Chemical and genetic biosensors, targeted to different subcellular compartments, have been developed and continuously improved to monitor Ca dynamics in living cells. Here we describe the usage of Förster resonance energy transfer (FRET)-based Cameleon probes to investigate Ca influx across the plasma membrane (PM) or Ca release from the main intracellular Ca store, the endoplasmic reticulum (ER).
钙离子（Ca）是一种普遍存在的细胞内信使，能够产生多种细胞内信号，几乎调节每种细胞类型中的大量功能。针对不同亚细胞区室的化学和遗传生物传感器已经得到开发并不断改进，以监测活细胞中的钙动态。在这里，我们描述了基于荧光共振能量转移（FRET）的钙敏荧光蛋白探针的用法，以研究钙离子通过质膜（PM）的内流或从主要细胞内钙库内质网（ER）的钙释放。
