Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015, Zhejiang, People's Republic of China.
Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015, Zhejiang, People's Republic of China.
Genome Biol. 2019 Jan 23;20(1):19. doi: 10.1186/s13059-019-1624-4.
Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development.
Trim-Away 是一种从任何细胞类型中快速耗尽蛋白质的新技术。在抗体的引导下，TRIM21 选择要破坏的蛋白质。然而，该方法在模式生物中的适用性尚未得到研究。在这里，我们表明 Trim-Away 可以在斑马鱼胚胎中降解蛋白质。Trim-Away 比 morpholinos 更快地耗尽蛋白质，这使得能够在早期胚胎发生期间分析蛋白质的功能。此外，Trim-Away 可用于评估母体贡献的蛋白质在斑马鱼胚胎中的作用。我们的研究结果表明，Trim-Away 是在斑马鱼发育过程中对蛋白质进行功能分析的有力工具。
