Chen Wen-Xue, Li Jin-Gao, Wan Xiang-Hui, Zou Xue-Sen, Qi Shu-Yi, Zhang Yu-Qing, Weng Qiu-Min, Li Jun-Yu, Xiong Wen-Min, Xie Chen, Cheng Wei-Liang
Department of Clinical Laboratory, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China.
Department of Radiotherapy, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China.
Oncol Lett. 2019 Feb;17(2):1581-1588. doi: 10.3892/ol.2018.9771. Epub 2018 Nov 28.
The aim of the present study was to discuss the design of a microfluidic chip consisting of columns, and its use for the enrichment of nasopharyngeal cancer (NPC) cells. A microfluidic chip experiment was simulated using FLUENT software. Within the microfluidic chip, aptamers were bound to the reaction chamber (consisting of columns) using a biotin-avidin system. Cell suspension was introduced into the reaction chamber to capture NPC cells. NPC cells were subsequently eluted, and the capture rate of the cells was calculated. The modified aptamer-bound microfluidic chip was able to capture NPC cells with a capture rate of ~90%. The modified aptamer-bound microfluidic chip has a wide range of potential applications for the diagnosis of NPC.
本研究的目的是探讨一种由柱体组成的微流控芯片的设计及其用于富集鼻咽癌（NPC）细胞的用途。使用FLUENT软件模拟了微流控芯片实验。在微流控芯片内，适配体通过生物素-抗生物素蛋白系统与反应室（由柱体组成）结合。将细胞悬液引入反应室以捕获NPC细胞。随后洗脱NPC细胞，并计算细胞的捕获率。修饰后的结合适配体的微流控芯片能够以约90%的捕获率捕获NPC细胞。修饰后的结合适配体的微流控芯片在NPC诊断方面具有广泛的潜在应用。
