Xiong Wei, Wang Li, Yu Fenglei
Department of Thoracic Surgery, First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China.
Department of Thoracic Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
Oncol Lett. 2019 Feb;17(2):1946-1952. doi: 10.3892/ol.2018.9781. Epub 2018 Nov 30.
The present study investigated the expression and clinical significance of bone morphogenetic protein 6 (BMP-6) in patients with non-small cell lung cancer (NSCLC). The tumor and adjacent normal lung tissues were harvested from 65 patients with NSCLC. BMP-6 mRNA expression was measured by reverse transcription-quantitative polymerase chain reaction, while protein expression was measured using immunohistochemistry or an ELISA. Cell viability was determined using Cell Counting Kit-8. The association of BMP-6 mRNA expression with the prognosis of patients with NSCLC was analyzed using the Kaplan-Meier plotter database. BMP-6 mRNA expression in NSCLC tumor tissues was significantly reduced, compared with the adjacent normal lung tissues (P<0.001), yet no significant differences were observed between patients with different clinicopathological features (P>0.05). The expression level of BMP-6 protein in NSCLC tumor tissues was significantly reduced, compared with the adjacent normal lung tissues (P<0.05). Analysis with the Kaplan-Meier plotter database revealed that patients with NSCLC with low BMP-6 mRNA expression had a reduced overall survival rate (P<0.01). The active BMP6 protein significantly inhibited cell proliferation in H460, H1299, A549 and H520 cells. In conclusion, BMP-6 is a tumor suppressor in lung cancer and loss of BMP-6 expression is significantly associated with a poor prognosis in patients with NSCLC.
本研究调查了骨形态发生蛋白6（BMP-6）在非小细胞肺癌（NSCLC）患者中的表达及临床意义。从65例NSCLC患者中获取肿瘤组织及相邻正常肺组织。采用逆转录定量聚合酶链反应检测BMP-6 mRNA表达，使用免疫组织化学或酶联免疫吸附测定法检测蛋白表达。使用细胞计数试剂盒-8测定细胞活力。利用Kaplan-Meier绘图仪数据库分析BMP-6 mRNA表达与NSCLC患者预后的相关性。与相邻正常肺组织相比，NSCLC肿瘤组织中BMP-6 mRNA表达显著降低（P<0.001），但不同临床病理特征患者之间未观察到显著差异（P>0.05）。与相邻正常肺组织相比，NSCLC肿瘤组织中BMP-6蛋白表达水平显著降低（P<0.05）。通过Kaplan-Meier绘图仪数据库分析发现，BMP-6 mRNA表达低的NSCLC患者总生存率降低（P<0.01）。活性BMP6蛋白显著抑制H460、H1299、A549和H520细胞的增殖。总之，BMP-6是肺癌中的一种肿瘤抑制因子，BMP-6表达缺失与NSCLC患者的不良预后显著相关。
