利用聚合酶逐步法将多个功能核苷酸单元特异性地整合到 DNA 中及其在监测 DNA 结构变化中的应用。

Site-specific incorporation of multiple units of functional nucleotides into DNA using a step-wise approach with polymerase and its application to monitoring DNA structural changes.

机构信息

Department of Bioactive Material Sciences, Chonbuk National University, South Korea.

出版信息

Chem Commun (Camb). 2019 Feb 14;55(15):2158-2161. doi: 10.1039/c8cc09444f.

Abstract

We have developed a new method, a step-wise approach with polymerase, for site-specific incorporation of multiple units of functional nucleotides into DNA to form hairpin secondary structures. The fluorescence of the resulting DNA incorporating the functional nucleotides varied upon transitioning from single-strand to hairpin and duplex structures.

摘要

我们开发了一种新方法，利用聚合酶的逐步方法，将多个功能核苷酸单元特异性地掺入 DNA 中，形成发夹二级结构。掺入功能核苷酸的 DNA 的荧光在从单链到发夹和双链结构转变时发生变化。