Castaing-Cordier Thomas, Bouillaud Dylan, Bowyer Paul, Gonçalves Olivier, Giraudeau Patrick, Farjon Jonathan
CEISAM UMR CNRS 6230, Faculté des Sciences et Techniques 2 rue de la Houssinière, BP 92208, 44322, Nantes cedex 3, France.
Université de Nantes, GEPEA, UMR CNRS 6144 Bât CRTT, 37 boulevard de l'Université, BP406, 44602, St Nazaire cedex, France.
Chemphyschem. 2019 Mar 4;20(5):736-744. doi: 10.1002/cphc.201801116. Epub 2019 Feb 13.
Benchtop NMR spectrometers experience a great success for a wide range of applications. However, their performance is highly limited by peak overlaps. Emerging "pure-shift NMR" (PS NMR) methods have been intensively used at high field to enhance the resolution by homodecoupling strategies. Here, different PS methods have been implemented on a compact NMR spectrometer operating at 43 MHz. Among the PS methods, the recent PSYCHE scheme appears more sensitive than Zangger-Sterk (ZS) experiments and offers a substantial resolution improvement as compared to 1D H. On the other hand, despite their slightly lower sensitivity, ZS methods are more efficient to reduce broad signals and are more immune to strong couplings. Finally, the classical J-resolved pulse sequence is more efficient to reduce larger signals for bigger-sized molecules. The three approaches appear relevant for benchtop NMR and their combination forms an efficient toolbox to analyze a great diversity of samples.
台式核磁共振波谱仪在广泛的应用中取得了巨大成功。然而，它们的性能受到峰重叠的严重限制。新兴的“纯位移核磁共振”（PS NMR）方法已在高场中大量使用，通过同核去耦策略提高分辨率。在此，不同的PS方法已在一台运行于43 MHz的紧凑型核磁共振波谱仪上实现。在PS方法中，最近的PSYCHE方案似乎比赞格-斯特克（ZS）实验更灵敏，并且与一维氢谱相比，分辨率有显著提高。另一方面，尽管ZS方法的灵敏度略低，但它们在减少宽信号方面更有效，并且对强耦合更具抗性。最后，经典的J分辨脉冲序列对于减少较大尺寸分子的较大信号更有效。这三种方法对于台式核磁共振似乎都很适用，它们的组合形成了一个有效的工具箱，可用于分析各种各样的样品。
