Department of Anatomy, College of Biomedical Sciences, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
PLoS One. 2019 Jan 24;14(1):e0210670. doi: 10.1371/journal.pone.0210670. eCollection 2019.
Oxidative stress has been considered as one of pathogenesis of brain damage led by epilepsy. Reducing oxidative stress can ameliorate brain damage during seizures. However, expression levels of important antioxidative enzymes such as thioredoxin-1 (TRX1), thioredoxin-like 1 protein (TXNL1) and thioredoxin reductase 1 (TXNRD1) during seizures have not been investigated. In this study, we examined protein and mRNA expression levels of TRX1, TXNL1 and TXNRD1 in different brain regions in PTZ induced seizure model mice. We found that protein expression levels of TRX1, TXNL1 and TXNRD1 are simultaneously up-regulated by 2- or 3-fold in the cortex of both acute and chronic seizure model mice. But there is no unified expression pattern change of these enzymes in the hippocampus, cerebellum and diencephalon in the seizure model mice. Less extent up-regulation of mRNA expression of these enzymes were also observed in the cortex of seizure mice. These data suggest that antioxidative enzymes may provide a protective effect against oxidative stress in the cortex during seizures.
氧化应激被认为是癫痫引起的脑损伤的发病机制之一。减少氧化应激可以改善癫痫发作时的脑损伤。然而，在癫痫发作期间，重要抗氧化酶如硫氧还蛋白-1（TRX1）、硫氧还蛋白样蛋白 1（TXNL1）和硫氧还蛋白还原酶 1（TXNRD1）的表达水平尚未得到研究。在这项研究中，我们检查了 PTZ 诱导的癫痫模型小鼠不同脑区中 TRX1、TXNL1 和 TXNRD1 的蛋白和 mRNA 表达水平。我们发现，TRX1、TXNL1 和 TXNRD1 的蛋白表达水平在急性和慢性癫痫模型小鼠的皮质中同时上调了 2 到 3 倍。但在癫痫模型小鼠的海马、小脑和间脑，这些酶并没有统一的表达模式变化。在癫痫小鼠的皮质中，这些酶的 mRNA 表达也有较小程度的上调。这些数据表明，抗氧化酶可能在癫痫发作时为皮质提供对抗氧化应激的保护作用。
