基于全基因组测序的甲型流感病毒分型的两种工作流程评估。

Evaluation of two workflows for whole genome sequencing-based typing of influenza A viruses.

机构信息

Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland; Swiss Institute of Bioinformatics, Basel, Switzerland.

Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.

出版信息

J Virol Methods. 2019 Apr;266:30-33. doi: 10.1016/j.jviromet.2019.01.009. Epub 2019 Jan 21.

DOI:10.1016/j.jviromet.2019.01.009

PMID:30677464

Abstract

We compared two sample preparation protocols for whole genome sequencing of influenza A viruses. Each protocol was assessed using cDNA quantity and quality and the resulting mean genome coverage after sequencing. Both protocols produced acceptable result for samples with high viral load, whereas one protocol performed slightly better with limited virus count.

摘要

我们比较了两种用于流感 A 病毒全基因组测序的样品制备方案。每个方案均通过 cDNA 数量和质量以及测序后得到的平均基因组覆盖度进行评估。对于高病毒载量的样本，两种方案均得到了可接受的结果，而对于病毒载量有限的样本，一种方案的性能略好。