Esteban-Ballesteros Myriam, Sanchis Jaime, Gutiérrez-Corbo Camino, Balaña-Fouce Rafael, Rojo-Vázquez Francisco A, González-Lanza Camino, Martínez-Valladares María
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Grulleros 24346, León, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, León 24071, Spain.
Departamento de Parasitología, Universidad de la República (Regional Norte), Salto, Uruguay.
Res Vet Sci. 2019 Apr;123:153-158. doi: 10.1016/j.rvsc.2019.01.004. Epub 2019 Jan 4.
The present study was conducted to evaluate the in vitro anthelmintic activity and safety of methanolic and aqueous extracts of 9 plants against the ovine GIN Teladorsagia circumcincta. Initially the ovicidal efficacy of all extracts was tested at 50 mg/ml and at 1%, this last concentration for the methanolic extract of Elettaria cardamomum, by the Egg Hatch Assay. In those extracts with efficacy higher than 95%, the effective concentration required to inhibit 50% of egg hatching (EC) and their cytotoxicity, as the lethal dose 50 (LC), was also measured. The aqueous extracts of Aesculus hippocastanum, Isatis tinctoria, Chelidonium majus, E. cardamomum and Sisymbrium irio, with EC values ranging 1.30-2.88 mg/ml, and the aqueous extract of Jasminum polyanthum with a value of 6.41 mg/ml, showed the highest activities. The aqueous extract of J. polyanthum was the safest extract, followed by methanolic extract of E. cardamomum and aqueous extract of S. irio, all of them with a Selective Index higher than 1. According to our results, there is no correlation between the amount of total phenols or total tannins with the anthelmintic activity of the plants tested. Although results need to be interpreted with caution, as in vitro activity may not automatically translate into in vivo efficacy, those extracts with SI equal or higher than 1 and EC equal or lower than 6 mg/ml, could be taken into account for being used subsequently as feed or food additives in infected sheep.
本研究旨在评估9种植物的甲醇提取物和水提取物对绵羊胃肠道线虫捻转血矛线虫的体外驱虫活性和安全性。最初，通过虫卵孵化试验，在50mg/ml和1%（小豆蔻甲醇提取物的最后一个浓度）的条件下测试了所有提取物的杀卵效果。在那些驱虫效果高于95%的提取物中，还测定了抑制50%虫卵孵化所需的有效浓度（EC）及其细胞毒性，即致死剂量50（LC）。七叶树、菘蓝、白屈菜、小豆蔻和伊朗芥的水提取物，其EC值在1.30-2.88mg/ml之间，以及多花素馨的水提取物，其值为6.41mg/ml，表现出最高的活性。多花素馨的水提取物是最安全的提取物，其次是小豆蔻的甲醇提取物和伊朗芥的水提取物，它们的选择性指数均高于1。根据我们的结果，受试植物中总酚或总单宁的含量与驱虫活性之间没有相关性。尽管结果需要谨慎解释，因为体外活性可能不会自动转化为体内疗效，但那些选择性指数等于或高于1且有效浓度等于或低于6mg/ml的提取物，可考虑随后用作感染绵羊的饲料或食品添加剂。
