College of Life Sciences and Medicine, Zhejiang Sci-Tech University, China.
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, China.
Bioresour Technol. 2019 Apr;278:43-50. doi: 10.1016/j.biortech.2019.01.022. Epub 2019 Jan 8.
Current work proposes an innovative wheat straw biomass utilization strategy that connects efficient lignocellulose biodegradation with exo-polysaccharide (EPS) production in I. obliquus under submerged fermentation. The addition of Tween 80 increased the activities of ligninolytic enzymes MnP, LiP and Lac by 1200%, 125% and 39.9%, respectively. When wheat straw lignin recalcitrance was substantially reduced with the aid of Tween 80, I. obliquus was capable of utilizing the substrates and in turn accumulated EPS. The degradation of cellulose, hemicellulose and lignin reached 46.1%, 46.4% and 44.1% on Day 9 of growth, respectively. Meanwhile, the maximum mycelial biomass and EPS production increased by 23.3% and 142.9%, respectively. The EPS had higher contents of sugar, protein, uronic acid, and mannose ratio, and higher antioxidant activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radicals.
目前的工作提出了一种创新的小麦秸秆生物质利用策略，即在浸没发酵中连接高效木质纤维素生物降解与外多糖（EPS）生产。吐温 80 的添加分别将木质素过氧化物酶 MnP、LiP 和漆酶的活性提高了 1200%、125%和 39.9%。当小麦秸秆木质素的抗降解性在吐温 80 的帮助下大大降低时，斜卧青霉能够利用这些底物并积累 EPS。在生长第 9 天时，纤维素、半纤维素和木质素的降解分别达到了 46.1%、46.4%和 44.1%。同时，最大菌丝体生物量和 EPS 产量分别提高了 23.3%和 142.9%。EPS 具有更高的糖、蛋白质、糖醛酸和甘露糖比例含量，以及对 2,2-二苯基-1-苦基肼（DPPH）、2,2-偶氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和羟基自由基的更高抗氧化活性。
