The College of Life Sciences, Northwest University, Xi'an 710069, China; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.
Institute of Military Cognitive and Brain Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:753-759. doi: 10.1016/j.msec.2018.12.088. Epub 2018 Dec 27.
Hepatitis c virus (HCV) infection is one of major causes for chronic liver diseases worldwide and could lead to death. Development of effective HCV vaccines is a powerful auxiliary method of existing treatments. Adjuvants are necessary for modern vaccines to promote immune responses. Among the various nanomaterials that have been developed, multihydroxylated fullerene (C(OH)) has been proved as an efficient adjuvant for human immunodeficiency virus DNA vaccine. Here, we utilized three types of HCV recombinant proteins as antigens to investigate the activity of C(OH) as a protein vaccine adjuvant. The proteins were carried by C(OH) in a way of surface adsorption and self-assemble encapsulation. C(OH) at a relatively low dose was sufficient to promote both humoral and cellular immune responses to HCV protein antigens and reduce the usage of antigen. These results demonstrated the positive adjuvant properties of C(OH) when applied to protein vaccines.
丙型肝炎病毒（HCV）感染是全球慢性肝病的主要病因之一，可导致死亡。开发有效的 HCV 疫苗是现有治疗方法的有力辅助手段。佐剂是现代疫苗促进免疫反应所必需的。在已经开发的各种纳米材料中，多羟基富勒烯（C（OH））已被证明是人类免疫缺陷病毒 DNA 疫苗的有效佐剂。在这里，我们利用三种 HCV 重组蛋白作为抗原，研究 C（OH）作为蛋白疫苗佐剂的活性。这些蛋白通过表面吸附和自组装包封的方式被 C（OH）携带。相对较低剂量的 C（OH）足以促进针对 HCV 蛋白抗原的体液和细胞免疫反应，并减少抗原的使用。这些结果表明 C（OH）在蛋白疫苗中的应用具有积极的佐剂特性。
