Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran.
Environ Sci Pollut Res Int. 2019 Mar;26(8):7863-7872. doi: 10.1007/s11356-019-04255-6. Epub 2019 Jan 24.
Six strains of Trichoderma spp. and Piriformospora indica have been studied for cadmium tolerance and bioaccumulation capacities by the poisoned food technique. A quantitative assay for Trichoderma spp. and P. indica was conducted in broth cultures supplemented with different cadmium concentrations (0-500 mg/l). In addition, the growth pattern of P. indica was determined by growing the fungus in a solid medium amended with eight concentrations of cadmium (0-200 mg/l). Generally, an increasing cadmium gradient in the culture medium suppressed the ability of fungi for cadmium accumulation. However, a negative relation was observed between the biomass production of fungi and cadmium uptake (q: mg/g biomass). Results showed that Trichoderma spp., especially T. simmonsii [UTFC 10063], are tolerant to cadmium toxicity and have a high ability to cadmium bioaccumulation. The biomass production of T. simmonsii [UTFC 10063] was significantly stimulated and increased by 46.1% when cadmium concentration increased from 0 to 125 mg/l. Moreover, 91.7 and 31.2% of cadmium removal was observed at 10 and 500 mg/l of the cadmium concentration, respectively. P. indica, however, showed a lower tolerance and removal efficiency for cadmium as compared with Trichoderma spp. Therefore, Trichoderma spp., especially T. simmonsii [UTFC 10063], can be exploited as potent bio-removal agents in cadmium-polluted aqueous solutions. Graphical abstract.
六种木霉属和离蠕孢属菌株已通过毒饵技术研究了其对镉的耐受性和生物累积能力。在添加不同镉浓度（0-500mg/l）的培养液中对木霉属和离蠕孢属进行了定量分析。此外，通过在添加了 8 种镉浓度（0-200mg/l）的固体培养基中培养真菌来确定离蠕孢属的生长模式。通常，培养基中镉梯度的增加会抑制真菌的镉积累能力。然而，真菌生物量的产生与镉的吸收（q：mg/g 生物量）之间存在负相关关系。结果表明，木霉属，特别是 T. simmonsii [UTFC 10063]，对镉毒性具有耐受性，并且具有较高的镉生物累积能力。当镉浓度从 0 增加到 125mg/l 时，T. simmonsii [UTFC 10063]的生物量产量显著增加了 46.1%。此外，在 10 和 500mg/l 的镉浓度下，分别观察到 91.7%和 31.2%的镉去除率。然而，与木霉属相比，离蠕孢属对镉的耐受性和去除效率较低。因此，木霉属，特别是 T. simmonsii [UTFC 10063]，可被开发为镉污染水溶液中的有效生物去除剂。
