Zeytinoğlu Ayşın, Terek Demet, Arslan Ayşe, Erensoy Selda, Altun Köroğlu Özge, Bozdemir Tuğba, Yalaz Mehmet, Ergör Serap Nur, Öğüt Mehmet Fatih, Kültürsay Nilgün
Ege University Faculty of Medicine, Department of Medical Microbiology, İzmir, Turkey.
Ege University Faculty of Medicine, Division of Neonatology, İzmir, Turkey.
Mikrobiyol Bul. 2019 Jan;53(1):53-60. doi: 10.5578/mb.67724.
Cytomegalovirus (CMV), is the most common cause among congenital infections and is the most seen etiology in long-term sensorineural hearing loss (SNHL) and neurological impairment. Congenital CMV infection (CCMV) was reported in 0.15-2.2% of live-borne neonates in studies from different countries. A significant proportion of infected infants are asymptomatic after birth and might only be detected by routine screening methods during the new born period. The aim of this study was to screen the saliva of live-born neonates with areal-time PCR based method for the detection of CCMV in our hospital. Saliva samples collected in half an hour after birth by dry dacron swabs and were evaluated for CMV DNA (Rt-PCR, Abbott Molecular USA) from 1000 babies born in Ege University Faculty of Medicine Hospital Obstetrics Clinic between October 2015-October 2017. For the confirmation of CCMV, saliva positive newborns were evaluated with the same method for CMV DNA from their urine or blood within 21 days. All newborns were screened for sensorineural hearing tests. Subjects were 497 girls (49.7%) and 503 boys (50.3%), with a mean weight of 3116.8 g and mean of 37.61 birth week. CMV DNA was positive in the saliva of 16 newborns (1.6%). Fourteen newborns were weakly positive for CMV DNA in their saliva and were not confirmed for CCMV infection. Congenital CMV was confirmed in only two (0.2%) with the CMV DNA results in urine and/or blood samples. One of the two newborns with CCMV was symptomatic and had a neurosensorial hearing loss. The other one was asymptomatic. Saliva samples, taken immediately after birth with a noninvasive and easy method for the detection of CMV DNA is very important for diagnosis of CCMV. Positive samples should be confirmed with CMV DNA in urine or blood samples of these newborns. In this study, detection of positivity in saliva samples that were confirmed with other samples of our newborn population for CCMV was 0.2%. The specific diagnosis for CCMV in newborns with a noninvasive and easy collecting sample is important to avoid sequelae and for public health concerns.
巨细胞病毒（CMV）是先天性感染最常见的病因，也是导致长期感音神经性听力损失（SNHL）和神经功能损害最常见的病因。不同国家的研究报告显示，先天性CMV感染（CCMV）在活产新生儿中的发生率为0.15%-2.2%。相当一部分受感染的婴儿出生后无症状，可能仅在新生儿期通过常规筛查方法被检测到。本研究的目的是采用基于实时PCR的方法对我院活产新生儿的唾液进行筛查，以检测CCMV。在2015年10月至2017年10月期间，从伊兹密尔大学医学院医院妇产科诊所出生的1000名婴儿中，用干涤纶拭子在出生后半小时内采集唾液样本，并对其进行CMV DNA检测（实时荧光定量PCR，美国雅培分子公司）。为确诊CCMV，对唾液检测呈阳性的新生儿在21天内用同样的方法检测其尿液或血液中的CMV DNA。所有新生儿均接受感音神经性听力测试筛查。研究对象包括497名女孩（49.7%）和503名男孩（50.3%），平均体重为3116.8克，平均孕周为37.61周。16名新生儿（1.6%）的唾液中CMV DNA呈阳性。14名新生儿唾液中的CMV DNA弱阳性，未确诊为CCMV感染。仅2名（0.2%）新生儿的尿液和/或血液样本中的CMV DNA检测结果确诊为先天性CMV感染。两名患有CCMV的新生儿中，一名有症状，患有神经感觉性听力损失，另一名无症状。出生后立即采集唾液样本，采用非侵入性且简便的方法检测CMV DNA，对CCMV的诊断非常重要。阳性样本应通过这些新生儿尿液或血液样本中的CMV DNA进行确诊。在本研究中，通过我们新生儿群体的其他样本确诊的唾液样本中CCMV阳性率为0.2%。采用非侵入性且易于采集样本的方法对新生儿CCMV进行特异性诊断，对于避免后遗症和公共卫生问题非常重要。
