da Cunha Leal Plinio, Rey Moura Ed Carlos, Jorge Dino Cossetti Rachel, Ramos do Nascimento Johnny, Portela Bogéa Serra Izabel Cristina, de Paulo Ribeiro Bruno, Álvares Marques Vale Andre, Silva de Azevedo Dos Santos Ana Paula, Fernandes do Nascimento Flavia Raquel, Kimiko Sakata Rioko
Federal University of Maranhão, São Luís, MA, Brazil.
Rua das Boninas, Bloco 2, apartment 1202, condomínio Ile Saint Louis, São Luís, MA, 65077-552, Brazil.
BMC Res Notes. 2019 Jan 25;12(1):59. doi: 10.1186/s13104-019-4103-9.
The purpose of this study was to evaluate the effect of gabapentin on Ehrlich tumor growth in Swiss mice, a highly aggressive and inflammatory tumor model. Mice were grouped into sets of 5 animals and treated from days 2 to 8 with gabapentin 30 mg/kg body weight (G30) or 100 mg/kg body weight (G100), or normal sterile saline (control).
The mice were euthanized on day 10. Tumor growth, tumoricidal agents and inflammatory cytokines levels were assessed. At day 10, G30 and G100 mice gained weight, but there were no differences in tumor cell count or in ascites volume. In G100, there was a reduction in arginase and an increase in SOD activities. There was an increase in IL-6 and MCP-1 levels, especially in G100, but no alterations in TNF-α. There was no direct evidence of tumor induction by gabapentin. However, the findings suggest that its use modulates immune response to a more effector and less deleterious profile, with increase in activity of anti-oxidant enzymes and in cytokines that favor activation of macrophages, which could improve the general status of the tumor host.
本研究旨在评估加巴喷丁对瑞士小鼠艾氏肿瘤生长的影响，该肿瘤模型具有高度侵袭性和炎症性。将小鼠按每组5只进行分组，并在第2天至第8天用30mg/kg体重的加巴喷丁（G30）或100mg/kg体重的加巴喷丁（G100）或正常无菌盐水（对照组）进行治疗。
在第10天对小鼠实施安乐死。评估肿瘤生长、杀肿瘤剂和炎性细胞因子水平。在第10天，G30组和G100组小鼠体重增加，但肿瘤细胞计数或腹水量没有差异。在G100组中，精氨酸酶减少，超氧化物歧化酶（SOD）活性增加。白细胞介素-6（IL-6）和单核细胞趋化蛋白-1（MCP-1）水平升高，尤其是在G100组，但肿瘤坏死因子-α（TNF-α）没有变化。没有加巴喷丁诱导肿瘤的直接证据。然而，研究结果表明，其使用可调节免疫反应，使其向更具效应性和更少有害性的方向发展，抗氧化酶活性增加，有利于巨噬细胞激活的细胞因子增加，这可能改善肿瘤宿主的总体状况。
