Antioxidant and antimicrobial markers by UPLC-ESI-Q-TOF-MS of a new multilayer active packaging based on Arctostaphylos uva-ursi.

49 different non-volatile compounds were determined in Spanish Arctostaphylos uva-ursi leaves using UPLC-ESI-Q-TOF with MS technology. Both positive and negative electrospray ionization were applied. MarkerLynx was proposed as a powerful tool to distinguish samples from eight wild populations of Spain by determining their non-volatile markers. Development of HRMS methods let to analysis of metabolites in plants. Antioxidant and antimicrobial capacities of different extracts were evaluated. Plant extract with the strongest antioxidant and simultaneous good antimicrobial capacity (Lierta) was chosen and incorporated in a multilayer packaging. Then, antioxidant capacity of the new packaging was evaluated and the efficient free radical scavenging properties were demonstrated.