Tianjin Municipal Key Lab of Advanced Fiber and Energy Storage, School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin, China.
Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Pingjin Hospital Heart Center, Logistics University of PAPF, Tianjin, 300162, China.
Appl Biochem Biotechnol. 2019 Jul;188(3):769-786. doi: 10.1007/s12010-019-02959-6. Epub 2019 Jan 26.
As one of the typical phyllosilicate clays, hectorite (Hec) has some excellent characteristics and has been greatly applied in adsorption field for the removal of dye, endotoxin, etc. In this study, organic Hec nanocomposites modified with L-Lysine (Lys/Hec NCs) were prepared via solution intercalation method for BR removal. The effects of ionic strength, pH values, initial concentration of BR, and BSA concentration on the adsorption capacity for BR of Lys/Hec NCs were investigated. Results indicated that the adsorption capacity for BR of nanocomposites could reach 40 mg/g when the initial bilirubin concentration was 200 mg/L. However, the adsorption amount of Lys/Hec NCs decreased with increasing the concentration of BSA, but Lys/Hec NCs could still maintain a higher adsorption rate. The adsorption kinetics and adsorption isotherms indicated that the adsorption process of Lys/Hec NCs agreed well with the pseudo-second-order model and the Langmuir isotherm, respectively. Moreover, Lys/Hec NCs also exhibited excellent cytocompatibility. These obtained results demonstrate that Lys/Hec NCs prepared in this study had great potential to be used in hemoperfusion.
作为典型的层状硅酸盐黏土之一，海泡石（Hec）具有一些优异的特性，已在吸附领域得到了广泛应用，用于去除染料、内毒素等。本研究通过溶液插层法制备了经 L-赖氨酸（Lys/Hec NCs）修饰的有机海泡石纳米复合材料，用于去除 BR。考察了离子强度、pH 值、BR 初始浓度和 BSA 浓度对 Lys/Hec NCs 吸附 BR 的影响。结果表明，当初始胆红素浓度为 200mg/L 时，纳米复合材料对 BR 的吸附容量可达 40mg/g。然而，随着 BSA 浓度的增加，Lys/Hec NCs 的吸附量减小，但 Lys/Hec NCs 仍能保持较高的吸附速率。吸附动力学和吸附等温线表明，Lys/Hec NCs 的吸附过程分别符合准二级动力学模型和 Langmuir 等温线。此外，Lys/Hec NCs 还表现出良好的细胞相容性。这些研究结果表明，本研究制备的 Lys/Hec NCs 具有在血液灌流中应用的巨大潜力。
