评估商业免疫测定法在中国汉族系统性硬化症人群中的自身抗体。
Evaluation of a commercial immunoassay for autoantibodies in Chinese Han systemic sclerosis population.
机构信息
Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Department of Rheumatology and Clinical Immunology, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Peking Union Medical College Hospital, Beijing 100730, China.
出版信息
Clin Chim Acta. 2019 Apr;491:121-125. doi: 10.1016/j.cca.2019.01.020. Epub 2019 Jan 24.
OBJECTIVE
The autoantibody profile, which varies between races, has rarely been reported in Chinese Han systemic sclerosis (SSc) patients. The present study aims to investigate the autoantibody profile of Chinese Han SSc patients using a commercial line immunoassay.
METHODS
The prevalence of autoantibodies to Ro-52, PDGFR, Ku, PM75, PM100, Th/To, NOR90, Fib, RP155, RP11, CENP-B, CENP-A and Scl-70 were analyzed in serum samples obtained from healthy controls (n = 30), SSc patients (n = 320) and non-SSc connective-tissue disease patients (n = 100) using an Euroline Systemic Sclerosis Profile kit.
RESULTS
SSc patients had increased prevalence of anti-RP11 (P = 0.032), anti-CENPB (P < 0.001), anti-CENPA (P = 0.001) and anti-Scl-70 (P < 0.001), but had decreased prevalence of anti-Ro-52 (P = 0.004), when compared to non-SSc CTDs. Furthermore, SSc patients had increased prevalence of anti-Ro-52 (P = 0.003), anti-CENPB (P = 0.034), anti-CENPA (P = 0.034) and anti-Scl-70 (P < 0.001), when compared to HCs. In addition, SSc patients with interstitial lung disease (ILD) had increased prevalence of anti-Ro-52 (P = 0.046) and anti-Scl-70 (P = 0.035), but had decreased prevalence of anti-CENPB (P < 0.001) and anti-CENPA (P < 0.001). Moreover, SSc patients with pulmonary arterial hypertension (PAH) had increased prevalence of anti-Ro-52 (P = 0.013) and decreased prevalence of anti-Scl-70 (P = 0.001). Anti-Ro-52 and anti-Scl-70 had increased values for predicting ILD in SSc patients, while anti-Ro-52 had increased values for predicting PAH in SSc patients.
CONCLUSION
A commercial line immunoassay was used to evaluate the diagnostic value of autoantibodies in Chinese Han SSc patients. The results clearly indicate that the immunoblot assay has good diagnostic utility for SSc. Anti-Ro-52, anti-RP11, anti-CENPB, anti-CENPA and anti-Scl-70 are useful for diagnosing SSc in the Chinese Han population. Anti-Ro-52, anti-CENPB, anti-CENPA and anti-Scl-70 have a potential value for diagnosing ILD in SSc patients. Furthermore, anti-Ro-52 and anti-Scl-70 may be used to diagnose PAH in SSc patients.
目的
人种之间的自身抗体谱存在差异，这种差异在汉族系统性硬皮病（SSc）患者中鲜有报道。本研究旨在使用商业线免疫印迹法检测汉族 SSc 患者的自身抗体谱。
方法
采用 Euroline 系统性硬皮病谱试剂盒检测 30 例健康对照者（对照组）、320 例 SSc 患者和 100 例非 SSc 结缔组织病患者血清样本中 Ro-52、PDGFR、Ku、PM75、PM100、Th/To、NOR90、Fib、RP155、RP11、CENP-B、CENP-A 和 Scl-70 自身抗体的阳性率。
结果
与非 SSc CTD 患者相比，SSc 患者抗-RP11（P=0.032）、抗-CENPB（P<0.001）、抗-CENPA（P=0.001）和抗-Scl-70（P<0.001）的阳性率升高，而抗-Ro-52（P=0.004）的阳性率降低。与对照组相比，SSc 患者抗-Ro-52（P=0.003）、抗-CENPB（P=0.034）、抗-CENPA（P=0.034）和抗-Scl-70（P<0.001）的阳性率升高。此外，有间质性肺病（ILD）的 SSc 患者抗-Ro-52（P=0.046）和抗-Scl-70（P=0.035）的阳性率升高，而抗-CENPB（P<0.001）和抗-CENPA（P<0.001）的阳性率降低。此外，有肺动脉高压（PAH）的 SSc 患者抗-Ro-52（P=0.013）的阳性率升高，抗-Scl-70（P=0.001）的阳性率降低。抗-Ro-52 和抗-Scl-70 对 SSc 患者发生 ILD 的预测值升高，而抗-Ro-52 对 SSc 患者发生 PAH 的预测值升高。
结论
本研究采用商业线免疫印迹法评估了汉族 SSc 患者自身抗体的诊断价值。结果明确表明免疫印迹法对 SSc 具有良好的诊断实用性。抗-Ro-52、抗-RP11、抗-CENPB、抗-CENPA 和抗-Scl-70 对汉族 SSc 患者的诊断具有一定价值。抗-Ro-52、抗-CENPB、抗-CENPA 和抗-Scl-70 对 SSc 患者发生 ILD 具有潜在诊断价值。此外，抗-Ro-52 和抗-Scl-70 可能对 SSc 患者发生 PAH 具有诊断价值。
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