Tahiat Azzedine, Allam Ines, Abdessemed Amina, Mellal Yasmine, Nebbab Rachid, Ladjouze-Rezig Aicha, Djidjik Réda
Beni Messous University Hospital, Department of immunology ; Algiers Faculty of medicine, University of Algiers 1, Algiers, Algeria.
Specialized medical center of Ben Aknoun, Department of rheumatology, Algiers, Algeria.
Ann Biol Clin (Paris). 2020 Apr 1;78(2):126-133. doi: 10.1684/abc.2020.1532.
To describe the autoantibody profile in a cohort of Algerian patients with systemic sclerosis (SSc) and to determine clinical associations between SSc-related autoantibodies, disease subtypes and specific clinical features.
Consecutive Algerian patients with SSc were included in the present study. In addition to clinical characterization, all subjects underwent autoantibody testing using indirect immunofluorescence, immunoenzymatic, and line immunoblot assays.
A total of 150 patients were included in this study, 103 (68.7%) had limited cutaneous SSc (lcSSc), 42 (28%) had diffuse cutaneous SSc (dcSSc) and 5 (3.3%) had sine cutaneous scleroderma. One hundred thirty-five (90.0%) patients were positive for SSc-related autoantibodies, including 63 (42%) with more than one autoantibody. The two most frequent autoantibodies were anti-topoisomerase I (ATA) (76; 50.7%) and anti-SSA/Ro (49; 32.7%). Only 23 (15.3%) patients were positive for anticentromere; 9 (6%) were positive for anti RNA polymerase III; 5 (3.3%) for anti-U3 RNP; 3 (2%) for anti Th/To; 25 (16.7%) for anti-U1 RNP; 11 (7.3%) for anti-PM/Scl and 4 (2.7%) for anti-Ku. Anti-topoisomerase I was associated with dcSSc (p <0.0001), interstitial lung disease (ILD) (p <0.0001) and digital ulcers (p <0.0001). Anti-U3 RNP was associated with pulmonary arterial hypertension (PAH) (p=0.031).
Notable similarities and differences in the prevalence of SSc-related autoantibodies were found in our population when compared to other ethnic groups. ATA and anti-U3 RNP may be a reliable biomarker for ILD and PAH. Further studies should be conducted to better understand the ethnic influence on disease expression and autoantibody production.
描述一组阿尔及利亚系统性硬化症（SSc）患者的自身抗体谱，并确定SSc相关自身抗体、疾病亚型和特定临床特征之间的临床关联。
本研究纳入了连续的阿尔及利亚SSc患者。除了临床特征描述外，所有受试者均接受了间接免疫荧光、免疫酶法和线性免疫印迹分析进行自身抗体检测。
本研究共纳入150例患者，其中103例（68.7%）为局限性皮肤型SSc（lcSSc），42例（28%）为弥漫性皮肤型SSc（dcSSc），5例（3.3%）为无皮肤硬化型硬皮病。135例（90.0%）患者的SSc相关自身抗体呈阳性，其中63例（42%）有不止一种自身抗体。最常见的两种自身抗体是抗拓扑异构酶I（ATA）（76例；50.7%）和抗SSA/Ro（49例；32.7%）。只有23例（15.3%）患者着丝点抗体呈阳性；9例（6%）抗RNA聚合酶III呈阳性；5例（3.3%）抗U3 RNP呈阳性；3例（2%）抗Th/To呈阳性；25例（16.7%）抗U1 RNP呈阳性；11例（7.3%）抗PM/Scl呈阳性；4例（2.7%）抗Ku呈阳性。抗拓扑异构酶I与dcSSc（p<0.0001）、间质性肺病（ILD）（p<0.0001）和指端溃疡（p<0.0001）相关。抗U3 RNP与肺动脉高压（PAH）相关（p=0.031）。
与其他种族群体相比，我们人群中SSc相关自身抗体的患病率存在显著的异同。ATA和抗U3 RNP可能是ILD和PAH的可靠生物标志物。应进一步开展研究，以更好地了解种族对疾病表现和自身抗体产生的影响。
