Institute of Food Safety, Chinese Academy of Inspection & Quarantine, 11 Ronghua South Road, Beijing 100176, People's Republic of China.
J Food Prot. 2019 Feb;82(2):331-338. doi: 10.4315/0362-028X.JFP-18-221.
A multiflavor detection method, using gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS), has been developed for the simultaneous identification and quantification of eight prohibited flavor compounds in daily foods. Under the optimized extraction conditions, samples were purified directly through membrane filtration. Variables affecting the GC-MS/MS were optimized to obtain better separation. The excellent selectivity and sensitivity achieved in multiple reactions monitoring mode allowed satisfactory confirmation and quantitation. In this study, the linear ranges of the target compounds were 0.05 to 500 ng/L with good correlation coefficients ( R > 0.999). The limits of detection of target compounds ranged from 0.005 to 0.2 μg/kg. The average recoveries were in the range of 80.2 to 110.6% (beef jerky), 82.3 to 94.1% (cod liver oil), and 83.6 to 104.1% (candy).
建立了一种多味检测方法，采用气相色谱-三重四极杆串联质谱（GC-MS/MS），可同时鉴定和定量日常食品中的 8 种禁用香料化合物。在优化的提取条件下，样品直接通过膜过滤进行纯化。优化了影响 GC-MS/MS 的变量，以获得更好的分离效果。在多重反应监测模式下获得了出色的选择性和灵敏度，可进行令人满意的确证和定量。在本研究中，目标化合物的线性范围为 0.05-500ng/L，相关系数良好（R>0.999）。目标化合物的检出限范围为 0.005-0.2μg/kg。平均回收率在牛肉干（80.2-110.6%）、鱼肝油（82.3-94.1%）和糖果（83.6-104.1%）中的范围。
