College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing, 211816, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing, 211816, China.
J Biotechnol. 2019 Feb 20;292:39-49. doi: 10.1016/j.jbiotec.2019.01.010. Epub 2019 Jan 26.
Metal-driven papain-surfactant nanocomposite (PA@MSNC), a novel soft nanobiocatalyst, was successfully prepared via one-pot self-assembly technique in aqueous solution for the biosynthesis of N-(benzyloxycarbonyl)-L-alanyl-L-glutamine (Z-Ala-Gln) dipeptide in deep eutectic solvents (DESs). The metal-driven self-assembly process generated PA@MSNC as nanospheres of ˜130 nm in diameter, with high protein loading and relative enzyme activity of 420 mg/g and 80% (4270 U/g protein), respectively. PA@MSNC showed high apparent substrate affinity and catalytic efficiency. The stability of PA@MSNC at high temperature and extreme pH was significantly higher than that of free PA. Catalysis efficiency for the biosynthesis of Z-Ala-Gln by PA@MSNC in choline chloride: glycerol reaction medium was 1.69-fold higher than that of free PA, achieving a high product yield of 75.7% within 4 h. PA@MSNC also showed better techno-economic performance. We propose that enzyme-surfactant nanocomposite via metal-driven dynamically reversible coordination interactions contribute simultaneously promotes catalytic flexibility and configurational stability. The generated PA@MSNC has potential practical implications for green synthesis of dipeptide in DESs.
金属驱动的木瓜蛋白酶-表面活性剂纳米复合材料（PA@MSNC）是一种新型的软纳生物催化剂，通过在水溶液中的一锅自组装技术成功制备，用于在深共熔溶剂（DESs）中生物合成 N-（苄氧羰基）-L-丙氨酰-L-谷氨酰胺（Z-Ala-Gln）二肽。金属驱动的自组装过程生成了直径约为 130nm 的 PA@MSNC 纳米球，具有高蛋白质负载量和相对酶活性，分别为 420mg/g 和 80%（4270U/g 蛋白质）。PA@MSNC 表现出高的表观底物亲和力和催化效率。PA@MSNC 在高温和极端 pH 下的稳定性明显高于游离 PA。在胆碱氯化物：甘油反应介质中，PA@MSNC 用于合成 Z-Ala-Gln 的催化效率比游离 PA 高 1.69 倍，在 4 小时内达到了 75.7%的高产物收率。PA@MSNC 还表现出更好的技术经济性能。我们提出，通过金属驱动的动态可逆配位相互作用的酶-表面活性剂纳米复合材料同时促进了催化灵活性和构象稳定性。生成的 PA@MSNC 对 DESs 中二肽的绿色合成具有潜在的实际意义。
