Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
Center of Healthy Ageing, University of Copenhagen, Copenhagen, Denmark.
Acta Ophthalmol. 2019 Sep;97(6):567-576. doi: 10.1111/aos.14021. Epub 2019 Jan 28.
To assess novel differences in serum levels of glucose, lactate and amino acids in patients with normal-tension glaucoma (NTG) compared to age-matched controls, at baseline and in response to universal hypoxia.
Twelve patients diagnosed with NTG and eleven control subjects underwent normobaric hypoxia for 2 hr. Peripheral venous blood samples were taken at baseline, during hypoxia and in the recovery phase. Serum glucose and lactate levels were measured by a blood gas analyser. Amino acids were analysed by high-performance liquid chromatography.
Baseline levels of lactate and total amino acids were significantly lower in patients with NTG compared to healthy controls. No differences were seen in blood glucose levels between the two groups. Lactate levels remained unchanged during hypoxia in the control group, but increased in patients with NTG. In the recovery phase, total amino acid levels were reduced in the control group, whereas no changes were found in patients with NTG.
Reduced serum levels of lactate and total amino acids were identified as potential markers for NTG. Moreover, significant differential regulatory patterns of certain amino acids were found in patients with NTG compared to control subjects. Overall, our results suggest a link between systemic energy metabolites and NTG and support a novel understanding of glaucoma as an inner retinal manifestation of a systemic condition.
评估正常眼压性青光眼（NTG）患者与年龄匹配的对照组在基础状态和对普遍缺氧的反应中血清葡萄糖、乳酸和氨基酸水平的新差异。
12 名被诊断为 NTG 的患者和 11 名对照受试者接受了 2 小时的常压低氧。在基线、缺氧期间和恢复阶段采集外周静脉血样。通过血气分析仪测量血清葡萄糖和乳酸水平。通过高效液相色谱法分析氨基酸。
与健康对照组相比，NTG 患者的基线乳酸和总氨基酸水平明显较低。两组之间的血糖水平没有差异。对照组的乳酸水平在缺氧期间保持不变，但 NTG 患者的乳酸水平增加。在恢复阶段，对照组的总氨基酸水平降低，而 NTG 患者则没有变化。
血清乳酸和总氨基酸水平降低被确定为 NTG 的潜在标志物。此外，与对照组相比，NTG 患者的某些氨基酸的调节模式存在显著差异。总的来说，我们的研究结果表明全身能量代谢物与 NTG 之间存在联系，并支持将青光眼作为一种全身疾病的视网膜内表现的新认识。
