BB大鼠中细胞毒性胰岛细胞表面抗体的存在与显性糖尿病发病率之间的相关性研究。

Studies on the correlation between the presence of cytotoxic islet cell surface antibody and the incidence of overt diabetes in BB rats.

作者信息

Tanguay K E, Yoon J W, Hart D A

机构信息

Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Alberta, Canada.

出版信息

Diabetes Res. 1988 Aug;8(4):171-5.

PMID:3069281

Abstract

Antibodies directed to the surface of islet cells are often present in the sera of both humans with insulin-dependent diabetes and the BioBreeding (BB) rat. These autoantibodies have been implicated in the disease process although the correlation between the presence of islet cell surface antibodies (ICSA) and diabetes is controversial. In this study the presence of cytotoxic islet cell surface antibody (ICSA) was examined in 65 BB rats from 60-150 days of age. Blood was collected every 7-10 days and sera were assayed by a 51Cr-release assay using rat insulinoma cells (RINm5F) as target cells. Onset of diabetes was determined by bi-weekly urinalysis. The incidence of diabetes was 51%. While 29% of the rats exhibited cytotoxic ICSA and diabetes, 22% were overtly diabetic without showing any measurable ICSA. An additional 22% showed ICSA but did not become diabetic in the time period studied. These results suggest no correlation between cytotoxic ICSA and diabetes in our BB rat colony.

摘要

针对胰岛细胞表面的抗体通常存在于胰岛素依赖型糖尿病患者以及生物繁殖（BB）大鼠的血清中。尽管胰岛细胞表面抗体（ICSA）的存在与糖尿病之间的相关性存在争议，但这些自身抗体已被认为与疾病进程有关。在本研究中，对65只60至150日龄的BB大鼠检测了细胞毒性胰岛细胞表面抗体（ICSA）的存在情况。每7至10天采集一次血液，使用大鼠胰岛素瘤细胞（RINm5F）作为靶细胞，通过51Cr释放试验检测血清。通过每两周一次的尿液分析确定糖尿病的发病情况。糖尿病的发病率为51%。虽然29%的大鼠表现出细胞毒性ICSA并患有糖尿病，但22%的大鼠明显患有糖尿病却未显示出任何可检测到的ICSA。另外22%的大鼠显示有ICSA，但在研究期间未患糖尿病。这些结果表明在我们的BB大鼠群体中，细胞毒性ICSA与糖尿病之间没有相关性。