利用HiSeq测序对二型花柱植物（报春花科）的30个微卫星标记进行特征分析。
Characterization of 30 microsatellite markers in distylous (Primulaceae) using HiSeq sequencing.
作者信息
Wang Xinjia, Zhong Li, Wu Zhikun, Sun Huaying, Wang Hong, Li Dezhu, Barrett Spencer C H, Zhou Wei
机构信息
Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany Chinese Academy of Sciences Kunming 650201 Yunnan People's Republic of China.
University of the Chinese Academy of Sciences Beijing 100049 People's Republic of China.
出版信息
Appl Plant Sci. 2019 Jan 3;7(1):e01208. doi: 10.1002/aps3.1208. eCollection 2019 Jan.
PREMISE OF THE STUDY
Microsatellite markers were developed for , a perennial distylous herb belonging to section (Primulaceae), to facilitate future investigations of the population genetics and mating patterns of populations in this species.
METHODS AND RESULTS
We developed 30 microsatellite markers for using HiSeq X-Ten sequencing and measured polymorphism and genetic diversity in a sample of 36 individuals from three natural populations. The markers displayed relatively high polymorphism, with the number of observed alleles per locus ranging from one to 19 (mean = 4.42). The observed and expected heterozygosity ranged from 0-1.000 and 0.083-0.882, respectively. Twenty-nine of the loci were also successfully amplified in homostylous var. .
CONCLUSIONS
The microsatellite markers we have identified in provide powerful tools for investigating patterns of population genetic diversity and the evolutionary relationships between heterostyly and homostyly in this species.
研究前提
为报春花科节的一种多年生二型花柱草本植物开发了微卫星标记，以便于今后对该物种种群的群体遗传学和交配模式进行研究。
方法与结果
我们利用HiSeq X-Ten测序技术为开发了30个微卫星标记，并在来自三个自然种群的36个个体样本中测量了多态性和遗传多样性。这些标记显示出相对较高的多态性，每个位点观察到的等位基因数从1到19不等（平均值 = 4.42）。观察到的杂合度和预期杂合度分别为0 - 1.000和0.083 - 0.882。其中29个位点在同型花柱变种中也成功扩增。
结论
我们在中鉴定出的微卫星标记为研究该物种的群体遗传多样性模式以及异型花柱和同型花柱之间的进化关系提供了有力工具。
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