Weng Binghuan, Xu Wei, Su Lan, Shen Min, Li Rong, Xu Xiaopeng, Li Lanjuan
Key Laboratory of Reproductive Genetics of the Ministry of Education, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2018 May 25;47(5):520-524. doi: 10.3785/j.issn.1008-9292.2018.10.12.
To establish a cell lines for quality control of prenatal genetic diagnosis.
The recombined SV40LTag-pcDNA3.1(-) vector was constructed and transfected by lipidosome into human amniotic fluid cells with common aneuploidy. Positive clones were screened by G418, and the immortality of transfected cell line was identified.
Cell line with karyotype of 46, XY, t(8;19)(q24.3;q13.1) from primary amniotic fluid cells was established. Karyotype analytical results indicated that the cell line at its 15th generation maintained the same karyotype of its primary cell.
Gene can lead to immortality of amniotic fluid cells, which contributes to preparing cell lines for internal and external quality control in prenatal genetic diagnosis.
建立用于产前基因诊断质量控制的细胞系。
构建重组SV40LTag-pcDNA3.1(-)载体，通过脂质体转染到具有常见非整倍体的人羊水细胞中。用G418筛选阳性克隆，并鉴定转染细胞系的永生化。
从原代羊水细胞建立了核型为46, XY, t(8;19)(q24.3;q13.1)的细胞系。核型分析结果表明，该细胞系第15代保持了原代细胞相同的核型。
基因可导致羊水细胞永生化，有助于为产前基因诊断的内部和外部质量控制制备细胞系。
